This is accomplished using a centrifugal microfluidic platform that can process raw biological samples (e.g., blood, sputum, urine,) in order to perform high-quality bead-based immunofluorescent assays. The invention uses a simple rotary motor and custom-designed plastic disc to perform the ...
Bead-based immunoassays, however, do not necessarily have to rely on optical detection (Fig.5) (de la Escosura-Muniz et al.2008). Since the beads are micron sized, the resistive pulse detection technique can be used, similar to the classical Coulter counting setup for cell-based assays (Se...
using ZnO-NW EμPADs with the previously determined parameters (the 2 mm radius WE #2, 8 h of ZnO NW growth, addition of 5 µg of GOx, and 50 s of incubation time). The calibration results from the spiked PBS reveal a linear range of 0−15 mM (Figure 6a), and the correspond...
Bead-based assays;Cytometric bead immunoassay;Particle manipulation Definition Bead-based microfluidic platforms may be used for either particle manipulation or bead-based sensing especially in cytometric bead-based immunoassays. Bead-based microfluidic platforms are often geared to biomedical research and diag...
patients’ blood. CF is an autosomal recessive disease and is also the most common severe genetic disease, found mainly in people of European descent with an incidence of approximately 1 in every 3000 live births worldwide [26–28,20]. The main characteristic of this pathology is the multi-...
Importantly, microfluidic immunoassays have demonstrated their potentials in promoting the development of POC detection in practical applications with a sample-to-answer format using portable equipment and simple operations [24–27]. However, the current microfluidic basically requires complex design and fabr...
We used AutoPAD to recreate several literature examples of paper-based microfluidic devices (e.g., paper microzone plates43 and three-dimensional immunoassays17) and demonstrated how AutoPAD could be used to generate design modifications with minimal user effort. We expect that the ease and rapid ...
A number of clinical biochemical studies such as blood gas, glucose/lactate/cholesterol, nucleic acid sequence analysis, proteins/peptides, combinatorial synthesis, toxicity monitoring, immunoassays, and forensic analysis are also focused areas for developing microfluidic biochips...
2.1.1. Blood Presumptive Tests A good presumptive test for blood should be sensitive, quick, simple, safe, and specific [19]. Blood presumptive tests should detect a certain blood component which cannot be commonly found in everyday environment/chemicals [20]. Most presumptive tests for blood ...
While immunoassays for SARS-CoV-2 are less common compared to NA amplification, they are still precise and efficient [85]. Therefore, adapting SARS-CoV-2 as a detection target into a device like the one developed by Phaneuf et al. would not be very difficult, as antibodies for SARS-CoV-...