This book covers the state-of-the-art research on molecular biology assays and molecular techniques enabled or enhanced by microfluidic platforms. Topics covered include microfluidic methods for cellular separations and single cell studies, droplet-based approaches to study protein expression and fo......
The gain in efficiency of MEBarcoding compared with other methods ultimately is only limited by the number of samples you can pool onto a single MiSeq lane. Objectives for this method High-throughput sequencing has radically transformed almost all facets of traditional molecular biology. At the ...
[Methods in Molecular Biology] Microchip Diagnostics Volume 1547 || FISH-in-CHIPS: A Microfluidic Platform for Molecular Typing of Cancer CellsMicrofluidics offer powerful tools for the control, manipulation, and analysis of cells, in ... V Taly,JL Viovy,S Descroix 被引量: 0发表: 2017年 加...
high-throughput analysis approaches that it can provide to molecular biology, clinical medicine, and biomedical sciences5,6,7. The main advantages presented by microfluidics include integration, miniaturization, automation, and low sample and reagent consumption. Microchip devices have been developed for a...
Microfluidics technology for systems biology research. Systems biology is a discipline seeking to understand the emergent of a biological system by integrative modeling of the interactions of the molecular elem... CJ Wang,A Levchenko - 《Methods in Molecular Biology》 被引量: 23发表: 2009年 Rapid...
Non-planar microfluidic devices and methods for transferring fluids between vessels and microfluidic devices are provided. The devices may be contoured to physically contact non-planar vessels, such as pipes, tubes, vials, or syringes to... CD Karp,SD O'Connor,VK Gustafson - US 被引量: 28发表...
Active methods have several remarkable advantages. On the one hand, these methods can control the mixing process with higher performance. On the other hand, they allow for flexible selection of parameters to apply a variety of experimental requirements. However, these advantages represent a high ...
For example, the conditional medium co-culture leverages the conditioned medium of endometrial cells to simulate the internal environment and facilitates the regulation of communication between cells [203,204]. Other methods, such as the climbing plate co-culture [205], have also shown promise in ...
14. High-throughput single-cell analysis methods such as flow cytometry, mass cytometry, and single-cell RNA sequencing (scRNA-seq) are ideal for identifying single cells in a comprehensive manner based on molecular information15,16, and these methods have already begun to transform our ...
The next step was to generate complete dermo-epidermal constructs and study them using confocal microscopy (Fig.9). These constructs were generated as described in “Steps for the generation of the bilayer tissue model inside the chip” of the “Materials and methods” using GFP-hFBS and GFP-...