or structures (e.g. sub-cellular organelles or nanofabricated structures) in a quantitative fashion into single cells. Unfortunately, traditional microinjection techniques are costly and time consuming. At present, microinjection is performed with expensive equipment consisting of a microscope for cell ...
Although glass microwell arrays generated by microengraving glass substrates have been around for a long time, one of the earliest uses of microwells in PDMS for single-cell isolation was performed by Rettig et al.34 in 2005. Similarly, microtrap arrays are often used to isolate individual ...
microfabrication methods enable scientists to manufacture hydrogel-based blocks,microparticles, and fibers that may directly or indirectly be used as a scaffold intissue engineering[29]. Cell-loadedpolyvinyl alcohol(PVA)/gelatin-based hydrogelmicrofiberswith enhanced viability and growth potential were creat...
Samuels: Droplet microfluidic technology for single-cell high-throughput screening, Proc. Nat. Acad. Sci. 106, 14195–14200 (2009) Google Scholar S. McNamara, Y.B. Gianchandani: On-chip vacuum generated by a micromachined Knudsen pump, J. Microelectromech. Syst. 14, 741–746 (2005) ...
Single cell transcriptome profiling has emerged as a breakthrough technology for the high-resolution understanding of complex cellular systems. Here we report a flexible, cost-effective and user-friendly droplet-based microfluidics system, called the Nadia Instrument, that can allow 3′ mRNA capture of...
In recent years, considerable attentions have been paid to single cell analysis based... J Handley - 《Analytical Chemistry》 被引量: 0发表: 2002年 Integration of single cell injection, cell lysis, separation and detection of intracellular constituents on a microfluidic chip A microfluidic system ...
Physical means also play an important role in artificial cell manipulation. Optical tweezers technology, with its high spatio-temporal control, can precisely localize single or multiple artificial cells and can also be combined with microfluidic cells to drive and measure the mechanical properties of li...
single- and dual-stranded DNA, the aperture must be of comparable dimensions since the current reduction upon translocation scales proportionally tod3/D4; wheredis the diameter of the cell or molecule andDis the diameter of the aperture. This in consequence requires nanometer-size apertures with a...
molecules Review Microfluidic-Based Single-Cell Study: Current Status and Future Perspective Haiwa Wu 1, Jing Zhu 2, Yao Huang 3, Daming Wu 3,4 and Jingyao Sun 3,5,* 1 Department of Pathology, College of Medicine, The Ohio State University, Columbus, OH 43210, USA; wu.680@osu.edu 2...
Last, we also discuss the technology and application trend for microfluidics based single-cell analysis. Micromachines 2019, 10, 104 3 of 31 2. Microfluidic Single-Cell Manipulation With the development of Microelectromechanical Systems (MEMS) technology, many micro-scale devices have been fabricated ...