The Luminex Human Discovery multiplex assay kit (Catalog Number: LXSAHM; R&D Systems, Inc, USA) was used for simultaneous measurement of cytokines IL-1β, IL-6, IL-8, TNF-α, and INF-γ in serum samples. Upon completion of each multiplex assay, the amounts of serum cytokines were analy...
Total genomic DNA was extracted from rumen fluid using the FastDNA™ Spin Kit for Soil (MP Biomedicals, USA) according to the manufacturer’s instructions. Concentration and purity of extracted DNA was determined with TBS-380 and NanoDrop2000, respectively. DNA extract quality was checked on 1% ...
Stool DNA was extracted using MagPure stool DNA KF kit B (no. MD5115-02B). DNA concentrations were estimated using Qubit (Invitrogen). The DNA library was constructed with 200 ng DNA as input. Shotgun metagenomic sequencing was then performed on the BGI-seq platform50 to generate at ...
The Nextera XT kit was used to generate libraries from amplified DNA. Normal- ized libraries were pooled and sequenced using the 2 Â 250 Miseq v2 cartridge. At the Biopolymers Facility Harvard Medical School, samples were prepped using NuGen's mRNA-seq kit (Illumina) into next-gen ...
Following consent, participants had an initial study visit, to have their baseline physiology assessed by height, weight, and blood pressure measurement, as well as provide a urine sample and receive the study kit and materials. Participants were asked to self-collect stool at home three times a...
Fragment size was verified with gel electrophoresis, and the purified PCR products were quantified using the 1X dsDNA High Sensitivity kit with a Qubit 2.0 fluorometer (Invitrogen, Life Technologies Inc., Carlsbad, CA) via Qubit fluorometer. PCR products were spin column purified using either a ...
According to the manufacturer’s instructions, DNA purification was achieved with a NucleoSpin gDNA clean-up kit (Macherey–Nagel GmbH and Co. KG, Düren, Germany). Nucleic acid concentrations were then measured using a NanoDrop (Thermo Fisher Scientific Inc.). If not processed immediately, ...
The total soil DNA was isolated from the previously prepared samples using an MN NucleoSpin kit (MN, Germany). The homogenization of the samples required by one of steps in the protocol was performed using a Precellys 24 homogenizer (Bertin Technologies, Montigny-le-Bretonneux, France), at 6500...
Microbiome FAQs What is the microbiome? The microbiome is the community of microorganisms (bacteria, archaea, fungi, viruses and eukaryotes) that can be found inside and on the human and animal body, as well as on plants and in the environment. ...
When your purchase is complete, everything you need for your at-home test will be delivered to your door. Use the activation code on the back of the test kit to activate your test online at Thorne.com and then complete your health profile. ...