对于MHC II类阻断实验,肿瘤细胞37℃在20毫克/毫升抗HLA DR,DP,DQ抗体(克隆TU39,从Biolegend公司)温育30分钟,或在相关同种型对照中温浴。然后,肿瘤细胞加入和没有附加洗涤的TIL(最终浓度在T细胞刺中激鸡尾酒约为0.5毫克/毫升)到共培养物中。进行聚合四聚体(肽-MHC多聚体的PE缀合并在内部产生HLA-A2限制性MART...
Anti-HLA-DR抗体同上1-10mgBiolegend307666(5mg)307667（25mg）5mg 4609；25mg 12038Ultra-LEAF Purified anti-human HLA-DR Antibody anti-HLA-DR - L243 (http://biolegend.com)其他抗体，anti-HLA-E\F\G,anti-HLA-DQ\DP同上1-10mgBiolegend301702（100μg）$127Purified anti-human HLA-DR, DP, DQ ...
AS01与抗原保持时空上的共定位对于佐剂的效果至关重要。 含铝佐剂的亚单位疫苗进入体内,要按照MHC II类途径提呈,诱导体液免疫,其仅能通过微弱的(本底水平的)交叉提呈产生极低水平的CTL。减毒活疫苗能自然感染进入胞内,其必然能诱导强的细胞免疫,从抗原提呈的角度讲,这...
(MOPC-173 for MHC-II and MR1) or IgG2bk (MCP11 for CD1d) control antibodies or a mixture of ULTRA-LEAF purified antihuman IL-12p40 (C8.9, 5 µg/ml) and anti IL-18 (W17071A, 5 µg/ml) or (Biolegend) for 30 min at 37°C to evaluate-dependency of the observed ...
27.在一些方面,hla ii类分子是dp1、dp2、dp3、dp4、dp5、 dp6、dp8或dp9等位基因。 28.在一些方面,所述dpβ链与参考hla ii类分子相比对cd4蛋白的亲和力增加,其中所述参考hla ii类分子包含的dpβ链包含(i) 在对应于seq id no:1的氨基酸残基112的位置处的亮氨酸和/或(ii) 在对应于seq id no:1的氨基酸...
根据不同产品类型,MHC四聚体定制服务细分为:MHC-I、 MHC-II 根据不同应用,本文重点关注以下领域:{{application_str}} 本文重点关注全球范围内MHC四聚体定制服务主要企业,包括:Tetramer、 Creative Biolabs、 MBL Life Science、 KACTUS、 immunAware、 BioLegend、 ProImmune、 百普赛斯集团、 广州好芝生物科技 ...
Antigen discovery technologies have largely focused on major histocompatibility complex (MHC) class I-restricted human T cell receptors (TCRs), leaving methods for MHC class II-restricted and mouse TCR reactivities relatively undeveloped. Here we present
The antibodies specific for PerCP MHCII (clone M5/114,15.2), Pacific Blue F4/80 (clone BM8), APC CD49b (clone DX5), FITC CD4 (clone RM4-5), Pacific Blue B220 (clone RA3-6B2) and PE/cy7 CD19 (clone 6D5) were purchased from Biolegend. V500 CD11b (clone M1/70), APC CD86...
Where indicated, the antibodies M5/114.15.2 (anti-MHCII, eBioscience), 16-10A1 (anti-CD80, BioLegend), and GL-1 (anti-CD86, BioLegend) were added to cultures at a concentration of 1 μg/ml. 11B11 (anti-IL-4, eBioscience) was incubated at a concentration of 10 μg/ml. Where ...
(TECs)1. In contrast, all other cell types are thought to produce MHCII only in the setting of inflammation, specifically in response to the cytokine interferon-γ (IFNγ)2. However, more recently, an increasing number of other cell types have been shown to express MHCII at homeostasis ...