We next screened TCR3898-2, which recognizes an epitope from CTAG1B (DR4+/CTAG1B121–130/VLLKEFTVSG), against CIITA+CTSS+ G5-target cells expressing six different HLA class II alleles, including the restricting HLA-DRB1*04:01 allele, transduced with the invariant chain fused human peptidome...
of the proteins, the membrane-spanning domains and the cytoplasmic domains, and how they function in the cell. The yellow and green spheres represent bound peptide antigens. Lower panel: three-dimensional ribbon diagram of the structure of MHC-1 (HLA-A2), as determined by X-ray ...
As shown in Figure 5, C33-A, SiHa and HeLa cells exhibited higher relative expression of MICA (1.79, 2.22 and 2, respectively) when compared with MICA mRNA levels of HaCaT cells (whose value was normalized to 1). For MICB, we observed in C33-A cells (2.5 fold increase over the ...
As shown in Figure 5, C33-A, SiHa and HeLa cells exhibited higher relative expression of MICA (1.79, 2.22 and 2, respectively) when compared with MICA mRNA levels of HaCaT cells (whose value was normalized to 1). For MICB, we observed in C33-A cells (2.5 fold increase over the ...
CC Voigt1 and S Sommer1 1Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany; 2Max Planck Institute of Ornithology, Vogelwarte Radolfzell, Radolfzell, Germany and 3Smithsonian Tropical Research Institute, Balboa, Ancon, Republic of Panama Genes of the major histocompatibility complex (M...
CFSE可偶联到胞内蛋白上,随着细胞分裂一 等认为机体对外来抗原 (病毒感染、人工免疫)侵入 代,CFSE平均分配到两个子代细胞中,导致荧光信 后产生的CD4T细胞与 MHC1I类分子的亲和力很 号减弱一半。这样随着 PBMC扩增,各连续代细胞 高,故很容易检测到四聚体阳性的细胞,而 自身抗原 以荧光强度的2倍递减为特征。当...
414: Cell-Matrix Interactions Influence Endothelial MHC Class II Expression Via SOCS1 and 3 UpregulationQuality reportingTiered networksPurpose Evidence is accumulating that differences in endothelial cell (EC) phenotype including immune functionality are regulated by basement membrane composition and structure...
(20/41 alleles), with 36% of alleles expressed that were present in individuals with both RNAseq and MHC genotype data (9/25 alleles). As with MHC-I exon 3, common alleles were more likely to be expressed (Fig.1b). Interestingly, the six relatively common MHC-IIB exon 2 alleles ...
2 cells (LV-ctrl) or MDCKII clone no. 2 cells that express HLA-DR (LV-HLA-DR) were infected with VSV-H18 at an MOI of 100 at 37 °C. After 1 h, cells were fixed and subjected to PLA using mouse anti-H18 and rabbit anti-MHC-II antibodies. Specific PLA dots obtained after...
M Average isoelectric point for the agglomerated heavy chain CDR3s of naive (peach) vs. GC (purple) B cells, stratified by whether they are predicted to be included in (I-Ab/b of chimeras 1 + 2 and I-Ad/d of chimera 3) or excluded from (I-Ad/d of chimera 1 + 2) ...