(5)每次从磁力架上取下1个柱子,置于相应的15 mL离心管中,在柱顶端加入2-5 mL PB,轻轻推动活塞,收集流出液2。重复该操作一次。流出液1和2于4℃、300 g离心5 min。 (6)以1 mL PBS-2%FCS缓冲液重悬,转移90 μL置于5 mL流式管(对于流出液1离心...
MHC genes are expressed to produce surface antigens on the cell membrane. ... The main difference between MHC class 1 and 2 is thatMHC class 1 molecules present antigens to cytotoxic T cells with CD8+ receptorswhereas MHC class 2 molecules present antigens to helper T cells with CD4+ rece...
不过,虽然NK细胞介导IFN感觉缺失细胞的杀伤,但其他MHC I类分子的表达,包括Qa-1b,可能存在其他的额外抑制效应群体:因为其抑制活性需要B6小鼠中H-2D1蛋白信号序列衍生的tap依赖肽(Qdm)的表达。而Tap1、Tap2或H2-D1等阻碍Qdm的基因丢失可能会造成Qa-1b活性增强。 ...
不过,虽然NK细胞介导IFN感觉缺失细胞的杀伤,但其他MHC I类分子的表达,包括Qa-1b,可能存在其他的额外抑制效应群体:因为其抑制活性需要B6小鼠中H-2D1蛋白信号序列衍生的tap依赖肽(Qdm)的表达。而Tap1、Tap2或H2-D1等阻碍Qdm的基因丢失可能会造成Qa-1b活性增强。 研究显示: NK细胞的减少对缺失Qa -1b的肿瘤治疗...
B cells induce tolerance by presenting endogenous peptide-IgG on MHC class II molecules via an IFN-gamma-inducible lysosomal thiol reductase-dependent pathway. J Immunol Jul 15 2008;181(2):1153-60.Su, Y., G. Carey, M. Maric, and D. W. Scott. 2008. B cells induce tolerance by ...
(2020). “Broad and strong memory CD4+ and CD8+ T cells induced by SARS-CoV-2 in UK convalescent COVID-19 patients” https://www.nature.com/articles/s41590-020-0782-6 Figure 1. memory phenotype and differentiation status of HLA-B*40:01 restricted NP322-331 (MEVTPSGTWL)-specific T ...
Fig. 2: The initiating autoreactive clone does not need to enter GCs to break tolerance. A Experimental setup. 1p = 1 part, 2p = 2 parts. Frequencies of B cells B, CD4 C, CD8 D T cells, and idiotype B cells E in blood, spleen, inguinal and mesenteric lymph nodes (IngLN...
During local T cell education, those T cells recognizing either autologous MHC or allogeneic MHC were equally depleted. We constructed C57BL/6-MHC and BALB/c-MHC dual immunocompatible mice via thymus vaccination of C57BL/6-MHC into the BALB/c thymus and observed long-term graft tolerance ...
b. Two overlapping, MHC I MS-detected 5’ uORFs in LUZP1 as an example of tissue-specific, overlapping nuORFs identified by hierarchical ORF prediction. uORF2 (pink) was predicted in the CLL clade, and not at the root. uORF1 (cyan) was predicted at the root and not in the CLL ...
(2020). “Broad and strong memory CD4+ and CD8+ T cells induced by SARS-CoV-2 in UK convalescent COVID-19 patients” https://www.nature.com/articles/s41590-020-0782-6 Figure 1. memory phenotype and differentiation status of HLA-B*40:01 restricted NP322-331 (MEVTPSGTWL)-specific T ...