To test whether Mettl3 and Mettl14 could compensate for each other in SSCs, we generated double-mutant mice. We found the homozygous mutants (Mettl3f/∆/Mettl14f/∆Vasa-Cre, hereafter referred to as Mettls-vKO) displayed similar phenotypes to the Mettl3 or Mettl14 single-mutants (...
Next, we utilized the conditional mutant mice to investigate the specific roles of Mettl3 and/or Mettl14 in regulating hematopoiesis and HSC self-renewal in adult bone marrow (see Supplementary dataS1for detail methods). At 6 weeks after pIpC treatment, the bone marrow cellularity of eitherMx1...
RanGAP1 fragments with mutant (Mut) or wild-type (WT) binding sites were subcloned into the psiCHECK2 dual-luciferase reporter vector (Hanbio, China) resulting in the following constructs: RanGAP1-WT, RanGAP1-Mut. And the plasmids were co-transfected into HCT116 cells with shMETTL3-1/2 mi...
B The dual-luciferase activity of the NEAT1 wild-type sequence (NEAT1-WT) and NEAT1 mutant sequence (NEAT1-MUT) in LX-2 cells transfected with miR-342 mimics or miR-342 inhibitor. C Relative NEAT1 expression level in Ago2 immunoprecipitates compared to IgG immunoprecipitates. D NEAT1 ...
(WT) mice was used as a template. Moreover, to assess the knockout efficiency of Mettl3 in adult liver, the main organs and tissues DNA, including the liver was subjected to PCR using the following primer pair to amplify 338bp Mettl3 mutant fragment, F1: GTGCTGTGCCTTTCTTAG, R1: AGC...
4C, D). The miR-124486-5 mimic reduced the relative luciferase activity of wild-type MAPK1, but had no significant effect on that of mutant MAPK1, indicating that miR-124486-5 targets and binds to the 3ʹ UTR of MAPK1 (Fig. 4E, F). Fig. 4 CircRNA3634 serves as a miR-124486-...
STM2457 是一种 RNA 甲基转移酶 METTL3 的抑制剂 (IC50=16.9 nM),具有选择性和口服活性。STM2457 可用于急性髓系白血病 (AML) 的研究。 规格价格库存数量 1 mg ¥ 834 现货 5 mg ¥ 1,709 现货 10 mg ¥ 2,915 现货 25 mg ¥ 4,710 ...
(J) Dual-luciferase report assay for the luciferase activity of wild-type or the mutant ACSL3 vector in LoVo cells after sh-METTL3 or sh-NC transfection. (K) MeRIP and qRT-PCR assays detecting the m6A content in ACSL3 after METTL3 knockdown in CRC cell lines. (L, M) The decay ...
RICTOR wild-type+ miR-192-5p overexpression group (transfected with RICTOR wild-type vector and miR-192-5p mimic plasmid), RICTOR mutant group (transfected with RICTOR mutant vector), and RICTOR mutant+ miR-192-5p overexpression group (transfected with RICTOR mutant vector and miR-192-5p mimic...
7P. In the dual luciferase reporter assay, miRNA mimics significantly suppressed luciferase activity in cells transfected with the wild-type MDM2 plasmid, whereas it did not affect luciferase activity in cells with the mutant-type MDM2 plasmid (Fig. 7Q). In summary, circGLIS3 regulates MDM2 ...