CpG island methylation is a common aberration frequently associated with gene silen... Kapitskaya,K.,Y.,... - 《Bulletin of Experimental Biology & Medicine C/c of Biulleten Eksperimentalnoi Biologii I Meditsiny》 被引量: 5发表: 2014年 Lung cancer : molecular pathology and functional ...
Pablo A. Manavella ResearchOpen Access27 Dec 2024Nature Structural & Molecular Biology P: 1-13 News and Comment X-specific methylation distorts sex Despite theoretical expectations of balanced 1:1 sex ratios between females and males, many dioecious plant species exhibit sex ratio distortions. A ...
The gDNA methylation level was detected using a methylation-specific dot blot assay. In this dot blot approach, a gDNA mixture containing the methylated DNA to be detected was spotted directly onto an N + membrane as a dot inside a previously drawn circular template pattern. Compared with other...
To generate a catalogue of putative regulatory regions in each cell type we applied a fragment-level analysis across all samples from each cell type, independently of other cell types. We scanned the entire genome and identified genomic regions in which at least 85% of DNA fragments with at le...
Determining standardized DNA methylation level, comprises: (a) quantitative determination of presence of transposon and/or its fragment in a genomic DNA, comprising amplification of non-bisulfited or bisulfited genomic DNA with a primer pair; (b) quantitative determination of existence of differentially...
(Youngblood et al., 2011). The CR-B and CR-C regions in non-tolerantT cells, which expressed a low level of PD-1, had significantly enriched 5-hydroxymethylcytosine (5hmC, a potential intermediate of 5 mC oxidation). In contrast, tolerated effector CD4+T cells with higher levels of PD...
(RT-PCR).Results 14 cases were found to have an increased methylation in the promoter region,especially in patients with high risk MDS.The P15 mRNA level was significantly decreased in patients with MDS(P0.05).A significant correlation existed between p15 methylation and mRNA decrease(P0.05)....
(Additional file1: Fig. S14b). When we examined this from a chromatin centric view, we found that the expression level of most genes with gain/increase of active marks were upregulated, whereas these with loss/decrease of active marks were down-regulated (Fig.6e). These results, together ...
133, 134, 135, 136, 137 The identification of cell of origin is essential to stem cell biology and cancer research. As an epigenetic memory of cell origin, DNA methylation profiles will be useful in the development of regenerative medicine and tumor-type-specific and patient type-specific ...
Self-collected samples from 593 HPV-positive women who participated in a primary HPV self-sampling trial (IMPROVE study; NTR5078), were tested for the DNA methylation markers ASCL1 and LHX8 using quantitative multiplex methylation-specific PCR (qMSP). The diagnostic performance for CIN3 and cervic...