The present disclosure provides systems and methods for controllable protein generation. According to some embodiments, the systems and methods leverage neural network models and techniques that have been developed for other fields, in particular, natural language processing (NLP). In some embodiments, ...
2023年4月20日,Nature Methods期刊在线发表了题为:Engineering of NEMO as calcium indicators with large dynamics and high sensitivity的研究论文 。 该研究成果由北京师范大学认知神经科学与学习国家重点实验室的章晓辉团队、生命科学学院的王...
2023年4月20日,Nature Methods 期刊在线发表了题为:Engineering of NEMO ascalciumindicators with large dynamics and high sensitivity 的研究论文 。 该研究成果由北京师范大学认知神经科学与学习国家重点实验室的章晓辉团队、生命科学学院的王友军团队与中国科学技术大学的唐爱辉团队合作完成。该研究开发构建了一类检测钙...
The Critical Assessment of protein Intrinsic Disorder prediction (CAID) experiment was established as a community-based blind test to determine the state of the art in prediction of intrinsically disordered regions and the subset of residues involved in binding. A total of 43 methods were evaluated...
Engineering of bidirectional, cyanobacteriochrome-based light-inducible dimers (BICYCL)s Bidirectional, cyanobacteriochrome-based light-inducible dimers (BICYCL)s enable optogenetic control of protein–protein interactions with green and red light, allowing multiplexing with existing blue light-controlled too...
Due to the intrinsic complexity of protein architecture at the microscopic level and the inadequacy of theoretical methods to predict protein reactivity (ie, folding, stability, and function), protein engineering has emerged as a valuable tool to investigate structure–stability–activity relationships in...
of more complex protein-RNA interaction functions and resulted in greater predictive performance compared to classical approaches. For instance, GraphProt [20], a method based on a Support Vector Machine (SVM), uses string-and graph-kernels to encode the primary and secondary structure of an RNA...
The CRISPR system has been eagerly adopted by many researchers for its ease of use: to edit a genome, all one needs are a Cas9 protein and a single guide RNA (sgRNA) with ∼20-nucleotide complementarity to the DNA target site. But this simplicity does not preclude optimization, as Keith...
select article PROMIS, global analysis of PROtein–metabolite interactions using size separation in Arabidopsis thaliana Research articleOpen access PROMIS, global analysis of PROtein–metabolite interactions using size separation in Arabidopsis thaliana Daniel Veyel, Ewelina M. Sokolowska, Juan C. Moreno, ...
When encapsulated bacteria expressing the protein of interest are solubilized, the resulting nanocontainer retains (that is, prevents the exit or loss of) the solubilized protein variant and its encoding gene but allows entry of a fluorescently labeled ligand. Detergent-stable variants of the examined...