predictiongenomeannotationSince the structure of the DNA molecule was identified half a century ago, the complete genome sequence has been determined for 37 prokaryotes and several eukaryotes. With the exponential growth of genetic information, bioinformatics has attempted to predict gene locations and ...
Clinically, they are gaining popularity as biomarkers or innovative therapeutics; to date there are more than 60 peptide drugs approved and more than 150 in clinical development. The emerging field of peptidomics comprises the comprehensive qualitative and quantitative analysis of the suite of peptides...
algorithms, but also with limitations that must be accounted for. While in silico tools have advanced research, their rapid development has also posed a conundrum of whether a single tool is preeminent or if multiple tools should be used. To realize the full potential of these in silico tools ...
This review was written in an attempt to present the reader with a decent spectrum of the available methods for gene function analysis that have been applied in the past decade. Knowledge emanating from the functional analysis of genes has applications in the fields of genetics and genomics, medi...
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First described in Escherichia coli, both MAGE and recombineering are being rapidly translated into diverse prokaryotes and even into eukaryotic cells. Together, this modern set of tools offers the promise of radically improving the scope and throughput of experimental biology by providing powerful new ...
which can determine the accessibility of ribosome binding sites and speed of local translation [54,55,56]. One seminal discovery in the field of synonymous variants was the observation that in the mutatedCFTRgene (c.1520_1522delTCT), which causes cystic fibrosis, a single synonymous variant (c...
Phylogenetic profiling encompasses an important set of methodologies for in silico high throughput inference of functional relationships between genes. The simplest profiles represent the distribution of gene presence-absence in a set of species as a seq
Comparative analysis of DNA and amino acid sequences is of fundamental importance in biological research, particularly in molecular biology and genomics. It is the first and key step in molecular evolutionary analysis, gene function and regulatory region prediction, sequence assembly, homology searching,...
The distance-based phylogenetic method is fast and remains the most popular one in molecular phylogenetics, especially in the big-data age when researchers often build phylogenetic trees with hundreds or even thousands of leaves. A distance-based method has two components: the evolutionary distance ma...