(60: 20: 20, acetonitrile: methanol: 0.1 M formic acid) and incubated for 10 min on ice to extract intracellular metabolites. After incubation, quenching solution was snap-frozen in liquid-nitrogen and lyophilized (−40 °C). Subsequently, dried samples were dissolved in ultra-pure ...
4). These results demonstrate that the acetonitrile is relatively stable under the reaction conditions and methanol is not originated from MeCN degradation. Fig. 2: Conversion of biomass resources to methanol. a Catalyst screening. Reaction conditions: 10 mg of glycerol, 10 mg of catalyst, 0...
The loading buffer was composed of water, 2% acetonitrile, and 0.1% trifluoroacetic acid. Peptides were eluted with mobile phase B gradient from 4 to 35% B in 60 min. Eluting peptide cations were converted to gas-phase ions by electrospray ionization and analyzed on a Thermo Orbitrap Fusion ...