It is found that in replacing acetonitrile for methanol, it is necessary to use two components of the mobile phase, containing only methanol and only formic acid, as on the joint presence of these substances, a prolonged retention drift is observed, presumably because of the acylation of ...
一个是混合编写的移动阶段850ml的nah2po4缓冲区0.025m与150毫升甲醇和移动阶段b准备的混合与150毫升850毫升acetonitrile甲醇。 翻译结果5复制译文编辑译文朗读译文返回顶部 流动相A通过混合850机器语言准备0.025M NaH2PO4缓冲与150机器语言甲醇,并且流动相B通过混合850机器语言准备乙腈与150机器语言甲醇。 相关内容 a为什么生...
Phase ratio Φ for an HPLC column is a common parameter being defined as the ratio between the volume of the stationary phase Vst and the void volume of the column V0. Although apparently simple, the evaluation of phase ratio presents difficulties because there is no sharp boundary between the...
After washing, filters were put into 8 mL precooled (−20 °C) quenching solution (60: 20: 20, acetonitrile: methanol: 0.1 M formic acid) and incubated for 10 min on ice to extract intracellular metabolites. After incubation, quenching solution was snap-frozen in liquid-nitrogen...
一个inertsilodsc-18、5μm列250×4.6mm内径在梯度模式,移动阶段,其中载有0.025米钠磷酸盐缓冲区:甲醇(85:15)和移动阶段b、包含acetonitrile:甲醇(85:15)是用在不同的时间间隔。 翻译结果5复制译文编辑译文朗读译文返回顶部 inertsil ODS C-18, 5 μm专栏有250×4.6毫米I.D。 在梯度方式下,当流动相A,包含...
column (ThermoFisher EasySpray Pepmap RSLC C18, 150 µm × 150 mm, 2 µm) and the peptides were eluted using a flowrate of 1.0 µL/min using 0.1% formic acid in water (buffer A) and 80% acetonitrile in buffer A (buffer B) as mobile phases for gradient elution...
Schure, Mobile phase effects in reversed- phase liquid chromatography: a comparison of acetonitrile/water and methanol/water solvents as studied by molecular simulation, J. Chromatogr. A 1218 (2011) 2203-2213.Jake L. Rafferty, J. Ilja Siepmann, and Mark R. Schure, "Mobile phase effects in ...
Mobile phase B was composed of acetonitrile and 0.1% formic acid. Samples were loaded onto the trap column (C18 PepMap100, 5 μm particle size, 300 μm × 5 mm, Thermo Fisher Scientific) for 4 min at 18 μL/min. The loading buffer was composed of water, 2% acetonitrile, and ...
Alcohol determination in beverages using polar capillary gas chromatography-mass spectroscopy and an acetonitrile internal standard. Concordia Coll. J. Anal. Chem. 2012, 3, 6–12. 33. Yaritaa, T.; Nakajima, R.; Otsuka, S.; Ihara, T.; Takatsu, A.; Shibukawa, M. Determination of ethanol...
Sweet potato has been found to be rich in polyphenolic compounds such as caffeic acid, quercetin and their derivatives [18]. Polyphenols are a group of phytochemicals which are considered highly potent antioxidant and bioactive compounds [21,22]. There are several literature evidence of the ...