3) 重组装Reassemble_bins:利用原始序列和评估软件二次组装,改善Bin的N50、完整度4) 定量Quant_bins: 估计样品中每个bin的丰度并热图展示5) 气泡图Blobology: blobplots可视化群体的contigs的物种和Bin分布6) 物种注释Classify_bins: 对Bin物种注释7) 基因注释Annotate_bins: 预测Bin中的基因 软件安装 系统要求 ...
metawrap classify_bins模块在NCBI_nt和NCBI_tax数据库基础上,使用工具Taxator-tk对每条contigs进行分类学注释,然后再估计bins的分类。注释结果的准确性也是由参考数据库决定的。 物种注释 步骤十 annotate_bins 分箱基因功能注释 bin_funct_annotations prokka完成的gff文件 bin_translatedgenes 翻译后的基因蛋白序列 bin...
metawrap quant_bins -b BIN_REFINEMENT/metaWRAP_bins -t 36 -o QUANT_BINS -a ASSEMBLY/final_assembly.fasta CLEAN_READS/ERR*fastq prokka注释 /home/wentao/Desktop/Shared_Folder/metage_pipe_Liu/bin_cs/BIN_REFINEMENT/metaWRAP_bins 这里提示我pr...
metaWRAP annotate_bins -o FUNCT_ANNOT -t 8 -b BIN_REASSEMBLY/reassembled_bins/ 每个bin基因注释的gff文件见FUNCT_ANNOT/bin_funct_annotations head FUNCT_ANNOT/bin_funct_annotations/bin.1.orig.gff NODE_75_length_31799_cov_0.983871 Prodigal:2.6 CDS 2866 3645 . - 0 ID=HMOHEJHL_00001;inference=...
解决:修改～/soft/anaconda3/envs/metawrap/bin/metawrap-modules/annotate_bins.sh 参考: https://github.com/bxlab/metaWRAP/issues/193 https://github.com/bxlab/metaWRAP/blob/master/bin/metawrap-modules/annotate_bins.sh 之后可能会遇到问题 This copy of tbl2asn is more than a year old. Please down...
The metaWRAP-Quant_bins module can be used to quickly estimate the abundance of each bin in each of the metagenomic samples. MetaWRAP-Classify_bins can be used to conservatively but accurately estimate their taxonomy. Finally, the bins can be functionally annotated with the metaWRAP-Annotate_bins ...
The metaWRAP-Quant_bins module can be used to quickly estimate the abundance of each bin in each of the metagenomic samples. MetaWRAP-Classify_bins can be used to conservatively but accurately estimate their taxonomy. Finally, the bins can be functionally annotated with the metaWRAP-Annotate_bins ...
and N50, and reduce contamination 4) Quant_bins: estimate bin abundance across samples 5) Blobology: visualize the community and extracted bins with blobplots 6) Classify_bins: conservative but accurate taxonomy prediction for bins 7) Annotate_bins: functionally annotate genes in a set of bins ...
quant_bins 量化每个 bin 在样本中的丰度 classify_bins 对基因组 bin 进行分类注释 annotate_bins 草图基因组的功能注释 --help | -h 显示此帮助信息 --version | -v 显示 metaWRAP 版本 --show-config 显示 metaWRAP 配置文件的存储位置 配置数据库 ...