The fertilization and developmental potentials of NPVS and non-NPVS oocytes were compared by calculating odds ratios using a mixed-effects logistic regression model. We also compared the embryo transfer outcomes of those used for single vitrified-warmed blastocyst transfer after developing into the ...
It is not known whether a correlation exists between model-predicted estimates of ovarian reserve and data generated by direct histological analysis of ovarian tissue. The aim of this study was to compare mean non-growing follicle density values obtained from analysis...关键词:...
However, since one pair of antiparallel microtubules would be sufficient to pull a chromosome to the pole, and it is possible that the kinetochore initiates the formation of one or two microtubules, for some cells, this model is still a contender. The sliding filament hypothesis has found ...
Intervention(s): Metaphase II oocytes were stripped of their cumulus cells, cooled directly to 0°C, and held for periods of 1 to 10 minutes. They were then fixed at 37°C, stained for immunofluorescence, and examined microscopically. Main Outcome Measure(s): Morphology of the meiotic spindle...
(ii) Eg5 generates local stresses throughout the structure and speckles are not affected in the pole region after the cut.b, Antiparallel overlaps in the DR that survive after the laser cut are the only ones that can drive the poleward speckle movement.c, Fluorescent speckle image before ...
We have previously demonstrated that this association between sister chromatid pairs is dependent on TopoIIα as post-extraction treatment of metaphase spreads with recombinant TopoIIα results in rescue of this phenotype to control levels2. Fig. 1: Phosphorylation of Aurora B S227 is required to ...
Maeshima K, Laemmli UK (2003) A two-step scaffolding model for mitotic chromosome assembly. Dev Cell 4:467–480 CASPubMed Martinez-Garcia M, Schubert V, Osman K, Darbyshire A, Sanchez-Moran E, Franklin FCH (2018) TOPII and chromosome movement help remove interlocks between entangled chromoso...
Metaphase chromosome structure: evidence for a radial loop model. Electron micrographs of thin sections of metaphase chromosomes isolated from HeLa cells provide new insight into the higher-order arrangement of the nucleoprotein fiber. Micrographs obtained from chromosomes swollen by chelation of the d.....
A new parameter of human metaphase chromosomes, the "relative thread gradient" is defined. It is the length of one winding of a chromatide relative to the total length of a normal, X-containing set. Furthermore a model of the human metaphase chromosome is discussed. In the cylindrical basic...
Figure 1c-ii and Supplementary Movie 1b also show the projection images of the 3D reconstruction of the chromosome virtually dissected by a tilted cutting plane. Careful observation of the 3D reconstructed chromosome revealed that it consisted of more or less evenly packed chromatin fibres in both...