Ultrafiltration is used for protein concentration and buffer exchange, both for the conditioning of the feed between other unit operations and in the final product formulation. However, actual protein purification, which typically refers to the separation of a desired protein product from other protein...
Purification and characterization of outer membrane protein P6, a vaccine antigen of non-typeable Haemophilus influenzae Karalus RJ, Murphy TF: Purification and characterization of outer membrane protein P6, a vaccine antigen of non-typeable Haemophilus influenzae. FEMS Immunol... RJ Karalus,TF Murphy...
Whether the target membrane protein is tagged, single or multi-pass transmembrane, single-protein or large-protein complex, we can perform small- or industrial-scale protein isolation and purification. On this research platform, customers can always find the optimal solution. ...
PurificationSMALPPolymerNanodiscDetergentMembrane proteins are localised within a lipid bilayer; in order to purify them for functional and structural studies the first step must involve solubilising or extracting the protein from these lipids. To date this has been achieved using detergents which ...
This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects...
Membrane Protein Purification and Crystallization 电子书 读后感 评分☆☆☆ 评分☆☆☆ 评分☆☆☆ 评分☆☆☆ 评分☆☆☆ 类似图书 点击查看全场最低价 出版者:Academic Press 作者:Jagow, Gebhard Von (EDT)/ Von Jagow, Gebhard (EDT)/ Schagger, Hermann (EDT)/ Hunte, Carole (EDT) 出品人: 页数:3...
Proprietary Technology in Membrane Protein Purification · Construct design and protein engineering of highly expressed and homogenous preparation of challenging membrane targets · Large scale (>10mgs) of soluble and high quality of membrane proteins applied for: ■ In vitro test ■ An immunizing anti...
Finally, the protein was loaded onto a Superdex 200 16/600 pg gel filtration column on an ÄKTA protein purification system. Size exclusion was performed at 1 ml.min−1 and 1 ml fractions analysed by SDS-PAGE. BAM-containing fractions were pooled and concentrated as above to 30–50...
Based on preliminary evidence indicating that a cell-associated protein of U937 (a human monocyte-like cell line) possessed cofactor activity and was not the C3b/C4b receptor, we sought to further characterize this protein. A sequential four-column purification procedure was devised that includes C3...
Most of our knowledge in this field comes from experimental cell biological data on specific proteins and from the results of computations on membrane protein structure. View chapterExplore book Membrane Proteins—Engineering, Purification and Crystallization Ankita Roy, in Methods in Enzymology, 2015 ...