Three types of hybridization are possible : DNA/DNA, RNA/RNA and RNA/DNA. System requirements Perl Filed under Compute enthalpy Entropy computation Calculate melting temperature Compute Calculate Computation Calculator What's new in MELTING 5.2.0: Added parameters for consecutive locked nucleic acids...
Calculator-controlled high-resolution melting measurements of bacterial DNAs: Basic information for taxonomy studiesNo Abstract available for this article.doi:10.1007/BF00481813L. PivecInstitute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha-6, CzechoslovakiaA. Vítek...
Optimization of the annealing temperature and concentrations of those primers and using melting curve analysis mainly sifted out the non-specificity issues of the SYBR green method. We then validated the assay with a total of 180 clinical samples where an equal number of samples were extracted ...
Based on the changes in fluorescence resulting from temperature shifts, melting curves—raw, normalised, and difference—are plotted. The plot shape depends on the amplicon length, sequence, GC content, and complementarity of the DNA strands [20]. Therefore, the sequence variations in the amplicon...