Higher temperatures cause progressively higher disruption of the hydrogen bonds between DNA strands; it is affected also by the base composition of the DNA because there are three hydrogen bonds...Springer NetherlandsEncyclopedia of Genetics Genomics Proteomics & Informatics...
探针法熔解曲线 探针法熔解曲线分析是一种利用特异性探针结合到目标DNA序列上的技术,随后通过对探针与目标DNA结合状态的温度依赖性检测,来识别和定量特定的DNA或RNA序列。这种方法的关键在于,当探针与目标序列完全匹配时,其熔解温度(Tm值)会较高;而如果存在单核苷酸多态性(SNPs)或其他变异,则会导致Tm值下降。因此,通...
2.Melting curveof target DNA fragments were drawn using real-time PCR through Light Cycler Instrument integrated with specific oligonucleotide probes,and the samples were genotyped according to the peak of the melting tempera.通过LightCycler实时PCR仪描绘扩增的目标DNA片段的熔解曲线,根据熔解温度(Tm)峰值判...
Results indicate that the presence of porphyrin changes the shape and parameters of DNA melting curve. The increase of porphyrins concentration results in the increase of the melting temperature (Tm) and the melting interval (T) of DNA. At the porphyrin-DNA concentration ratio r = 0.01, changes...
The relations between the melting curve and the denaturation maps are clarified. We observe that nearly always, the beginning and end of a gene melt at lower temperatures. The sharp features in the DM indicate that despite the long-range cooperative interactions, the DM do reflect the local ...
meltingcurve熔解analysisplasmidscarattoli METHODOLOGYARTICLEOpenAccess RapidplasmidreplicontypingbyrealtimePCR meltingcurveanalysis MaikelBoot,SusanneRaadsen,PaulHMSavelkoulandChristinaVandenbroucke-Grauls * Abstract Background:GenesencodingExtendedSpectrumBetaLactamasesareusuallylocatedontransferableplasmids. Eachplasmidco...
A high-resolution melt curve can be added to the end of the amplification run and analyzed immediately after completion Fast optimization: unlike DHPLC, thermal optimization is not required Low sample consumption: following HRM analysis, the ...
Melting curve assay and PCR microplate hybridization-ELISA assay should be further improved to increase their sensitivity and specialty.───熔解曲线法和PCR微板核酸杂交法需要进一步完善以提高敏感性和特异性。 Objective: To evaluate the feasibility and sensitivity of detecting the mutations of KRAS in co...
如果缓慢加热DNA溶液,并在不同温度测定其A260值,可得到“S”形DNA熔化曲线(melting curve)。从DNA熔化曲线可见DNA变性作用是在一个相当窄的温度内完成的。当A260值开始上升前DNA是双螺旋结构,在上升区域分子中的部分碱基对开始断裂,其数值随温度的升高而增加,在上部平坦的初始部分尚有少量碱基...
Wisplinghoff, Köln, Deutschland Synonym(e) Melting curve-Analyse Englischer Begriff melting curve analysis Definition Die Schmelzkurvenanalyse charakterisiert die Dissoziation doppelsträngiger DNA während der Erhitzung und ermöglicht die spezifische Detektion von Sequenzabwei- chungen bzw. ...