原因如下：采用Syber green的real-time PCR有可能扩增非特异性产物，光看PCR产物的信号无法区别到底是特异性产物还是非特异性的。由于扩增的序列已知，可以计算该序列的TM值，也就是加热到该温度，50%双链打开的。Syber green只有结合DNA双链才有荧光，所以PCR反应结束后，对产物进行加热，每加热一度，测...
In this study, a real-time PCR with melting curve analysis was established for molecular typing of V. parahaemolyticus . Eighteen large variably presented gene clusters (LVPCs) of V. parahaemolyticus which have different distributions in the genome of different strains were selected as targets. ...
we develop a novel analytical real-time PCR technique to detect multiple targets in a single fluorescence channel without melting curve analysis. In this technique, we show the intensity of the fluorescence signals of two discrete Tmtargets is different...
Monitoring polymerase chain reaction (PCR) once each cycle is a powerful method to detect and quantify the presence of nucleic acid sequences and has become known as & quote;real-time& quote; PCR. Absolute quantification of initial template copy number can be obtained, although quantification rela...
and Bottero, M.T., 2007. Identification of four tuna species by means of Real-Time PCR and melting curve analysis. Veterinary Research Communications , 31 ( Suppl. 1 ), 355–357关键词:ATPase 6 canned tuna melting curve Real-Time PCR species identification ...
And we also isolated Staphylococcus chromogenes a causative agent of exudative epidermitis in pigs and cattle from 3 samples. 展开 关键词: coa, mecA, Melting curve, Real time PCR, staphylococcus aureus 被引量: 1 年份: 2008 收藏 引用 批量引用 报错 分享 ...
Robert E. FarrellJr, in RNA Methodologies (Sixth Edition), 2023 Melting curve analysis The real-time PCR platform relies on an increasing level of fluorescence as an indicator of product accumulation. Those who fear amplicon contamination (should be everyone) feel very confident using real-time as...
1) Melting curve 熔解曲线 1. Influence of experimental conditions on probe melting curves based on fluorescence resonance energy transfer; 实验条件对荧光共振能量转移的探针熔解曲线影响 2. Melting curveof target DNA fragments were drawn using real-time PCR through Light Cycler Instrument integrated with...
摘要: We attempted to determine the benefits of three-channel multiplex real-time and melting curve analysis not only in detecting and distinguishing between nontuberculous (NTM) and the but also in identifying NTM to the species level.关键词: Humans Mycobacterium tuberculosis Mycobacterium Infections ...
2.Melting curveof target DNA fragments were drawn using real-time PCR through Light Cycler Instrument integrated with specific oligonucleotide probes,and the samples were genotyped according to the peak of the melting tempera.通过LightCycler实时PCR仪描绘扩增的目标DNA片段的熔解曲线,根据熔解温度(Tm)峰值判...