parahaemolyticus . In this study, a real-time PCR with melting curve analysis was established for molecular typing of V. parahaemolyticus . Eighteen large variably presented gene clusters (LVPCs) of V. parahaemolyticus which have different distributions in the genome of different strains were selected...
原因如下：采用Syber green的real-time PCR有可能扩增非特异性产物，光看PCR产物的信号无法区别到底是特异性产物还是非特异性的。由于扩增的序列已知，可以计算该序列的TM值，也就是加热到该温度，50%双链打开的。Syber green只有结合DNA双链才有荧光，所以PCR反应结束后，对产物进行加热，每加热一度，测...
Quantitative real-time PCR has revolutionized many aspects of genetic research, biomedical diagnostics and pathogen detection. Nevertheless, the full potential of this technology has yet to be realized, primarily due to the limitations of the threshold-based methodologies that are currently used for quan...
The aim of this study was to develop a LightCycler-based real time PCR (LC-PCR) assay and to evaluate its diagnostic use for the detection of Staphylococcus aureus in raw milk samples. Following amplification of 113 bp of coa gene encoding an coagulase precursor specific for Staphylococcus aureu...
During real-time PCR reaction, the duplex-driven fluorescence signal (designated as a yellow circle) of the probes depends on the temperature because of their melting curves (Fig. 1). Therefore, different intensities of those signals at certain temperatures at each cycle are able to detect the ...
Currently real time PCR is the most precise method by which to measure gene expression. The method generates a large amount of raw numerical data and processing may notably influence final results. The data processing is based either on standard curves or on PCR efficiency assessment. At the mom...
The repeatability and sensitivity of a simple, adaptable, semi-quantitative, realtime RT-PCR assay was investigated. The assay can be easily and rapidly applied to quantitate relative levels of any gene product without using standards, provided that amplification conditions are specific for the PCR ...
摘要: We attempted to determine the benefits of three-channel multiplex real-time and melting curve analysis not only in detecting and distinguishing between nontuberculous (NTM) and the but also in identifying NTM to the species level.关键词: Humans Mycobacterium tuberculosis Mycobacterium Infections ...
melting curve of the PCR products, indicating the specification of the present primers.The correlation coefficients of the standard for the Hairless and β-actin genes were 0.998 and 0.999, respectively, suggesting the strong linear relationship between the two groups.The results of repeatability ...
This real-time PCR assay with a SYBR Green post-PCR melting curve analysis targeting the polyphenolic adhesive protein gene developed was successfully applied to fresh, frozen and canned mussels, showing 100% of specificity, sensitivity and precision. Besides, results have demonstrated the applicability...