In this study, a real-time PCR with melting curve analysis was established for molecular typing of V. parahaemolyticus . Eighteen large variably presented gene clusters (LVPCs) of V. parahaemolyticus which have different distributions in the genome of different strains were selected as targets. ...
只有用Syber green的才要做，使用probe-primer的是不要做的。原因如下：采用Syber green的real-time PCR有可能扩增非特异性产物，光看PCR产物的信号无法区别到底是特异性产物还是非特异性的。由于扩增的序列已知，可以计算该序列的TM值，也就是加热到该温度，50%双链打开的。Syber green只有结合DNA双链...
we develop a novel analytical real-time PCR technique to detect multiple targets in a single fluorescence channel without melting curve analysis. In this technique, we show the intensity of the fluorescence signals of two discrete Tmtargets is different...
Monitoring polymerase chain reaction (PCR) once each cycle is a powerful method to detect and quantify the presence of nucleic acid sequences and has become known as & quote;real-time& quote; PCR. Absolute quantification of initial template copy number c
Applicability of real-time PCR combined with melting curve analysis for the study of hepatitis B virus genome mutations[J]. Hepatology, 2003,37(2) :478-479.Rodriguez-Frias F,Jardi R,Buti M,et al.Applicability of real-time PCR combined with melting curve analysis for the study of hepatitis...
Robert E. FarrellJr, in RNA Methodologies (Sixth Edition), 2023 Melting curve analysis The real-time PCR platform relies on an increasing level of fluorescence as an indicator of product accumulation. Those who fear amplicon contamination (should be everyone) feel very confident using real-time as...
Detection and characterization of Newcastle disease virus in clinical samples using real time RT-PCR and melting curve analysis based on matrix and fusion ... Aim: Newcastle disease is still one of the major threats for poultry industry allover the world. Therefore, attempt was made in this stud...
and Bottero, M.T., 2007. Identification of four tuna species by means of Real-Time PCR and melting curve analysis. Veterinary Research Communications , 31 ( Suppl. 1 ), 355–357关键词:ATPase 6 canned tuna melting curve Real-Time PCR species identification ...
Therefore, the methodology described in this study is useful for the authentication of mussel samples and could be easily applied as a fast routine food control laboratory test. 展开 关键词: Mytilus Species identification Real-time PCR Melting curve analysis Labelling ...
3.Detection of RSV A and B were based on determination of specific melting temperatures by real-time PCR and melting curve analysis using SYBR GreenⅠfluorescent dye.使用SYBR Green荧光染料结合熔解曲线分析进行检测,根据产物特征性熔解温度(melting temperatures Tm)鉴别RSV A、B亚型。英文...