(Bio-Rad) according to the manufacturer’s instructions on a CFX384 Touch Real-Time PCR Detection system (Bio-Rad). The gene copy number was calculated with Bio-Rad CFX Manager 3.1. PCR amplification of the coding regions ofActBandRplp0was used for normalization. The data from at least 6...
II. Meiosis Narrating things as they are means narrating them from the beginning, and even if I start the story at a point where the characters are multicellular organisms, for example the story of my relationship with Priscilla, I have first to define clearly what I mean when I say me an...
For example, Ptbp2, a key alternative-splicing regulator in the nervous system, is critical for male germ cell survival and male fertility through regulating the proper alternative splicing of germ cell messenger RNAs (mRNA) in the testis16. Rbm5, a male germ cell splicing factor, is ...
a Aggregated END-seq signal around SPO11 oligos, with hotspot example, showing absence of SPO11 central peak in Dmc1–/–. b Reduction in central signal at non-PAR X chromosome hotspots; aggregated signal on ChrX compared with all autosomes. c Aggregated END-seq signal around SPO11 oligos ...
(B) Example movie of meiosis II spindle assembly. (C) A single spindle forms in each meiosis II cell, but assembly results in spindles oriented parallel to each other (panel 1). Of all imaged cells (n = 75 total), only a single instance of non-parallel meiosis II spindles was ...