The invention relates to a double-target anti-tumor recombinant protein medicine OC001 for anti-tumor therapy, and construction and application thereof. The invention further provides application of the protein medicine to preparation of medicines for treating a variety of tumors.朱涛...
These compounds varied within different batch which could be overcame using recombinant protein technology [33].Synthetic materials are other choices which are easily produced and have excellent physical properties in coordination with medium to long-term biodegradability. However, their degradation can ...
Availability of data and materials The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.Abbreviations AD: Alzheimer’s disease AChE: Acetylcholine esterase Aβ: β-Amyloid AP-1-RE: Activator protein 1 response element CC:...
Some of the recombinant silk produced has been used for cell culture and biocompatibility studies with encouraging results [8]. However, for commercial applications more defined and reproducible properties of the polymerized material are needed. Also, if we could find a way to mimic the spider’s...
a recombinant production system based on yeast has been recently explored [59,60,61,62]. Some monodisperse gelatin-like polymers have also been obtained from recombinant protein biosynthesis systems based on engineered microorganisms. Gelatin is another combination of heterogeneous, animal-derived peptide...
Recombinant protein expression Recombinant 6xHIS tagged CEL and CEL-Tn (Clone ID: OHu22073C) was expressed in HEK293 and COSMC-KO HEK293-SC (simple cells)3and purified from Pro293 a Medium (Lonza, Basel, Switzerland) using Ni-NTA agarose (Qiagen). ...
Clemmons, D. R., Sleevi, M., Allan, G. & Sommer, A. Effects of combined recombinant insulin-like growth factor (IGF)-I and IGF binding protein-3 in type 2 diabetic patients on glycemic control and distribution of IGF-I and IGF-II among serum binding protein complexes.J. Clin. Endocr...
a recombinant production system based on yeast has been recently explored [59,60,61,62]. Some monodisperse gelatin-like polymers have also been obtained from recombinant protein biosynthesis systems based on engineered microorganisms. Gelatin is another combination of heterogeneous, animal-derived peptide...
A protein secretion vector plasmid for L. lactis, pNZ8148#2:SEC15, was used for recombinant gene expression. The sequence encoding mIL-1Ra optimized for L. lactis MG1363 was synthesized, and the sequence was cloned into pTAKN-2. L. lactis NZ9000 was purchased from MoBiTec (Goettingen, Germ...
Recombinant human CXCL13 (MCE, 1 μg/ml) was added to the bottom wells, B cells (1 × 105) or B cells (1 × 105) were pretreated with the CXCR5 antagonist ML339 (MCE, 40 μg/ml) for 30 min and placed in the upper wells. The cells were allowed to migrate for 12 h...