MTT assay was used to evaluate the inhibition of peanut albumin on 11 kinds of cancer cells. Compared with other cells, there was the great significant change in A549 cells. Morphological structures were detected by fluorescence microscope, and cell cycle was measured by flow cytometry (FCM). ...
Objective:To clarify the mechanism of artesunate(ART)inducing cells to apoptosis.Methods:Cells were cultured in vitro.MTT assay was undertaken to observe the growth inhibition effect of U937 cells exposed to artesunate. The artesunate-induced apoptosis in U937 cells was detected by cell morphological ...
mechanism of Fraction 6 on human leukemia multidrug resistance cells(K562/ADM) to empolder new reversal agent of tumor multidrug resistance.Methods: MTT assay was used to evaluate inhibitory effect of Fraction 6 alone,adriamycin(ADM) alone and Fraction 6 combined with adriamycin on proliferation of...
In order to evaluate the viability of RGC-5 in model group and Timosaponin B-II treatment group, we carried out MTT assay to detect cell viability. The statistical analysis of MTT result was shown in Figure 3. Comparing to normal control group (CTL), the cell viability was significantly dec...
MTT assay was used to evaluate cell viability for screening the optimal drug concentration of TPGs and TLPAs for Human Umbilical Vein Endothelial Cells (HUVEC) cells. As shown in Table 1, the viability of HUVEC cells ranged from 53.72 ± 2.99% to 101.61 ± 2.11% when treated ...
MTT assay showed the inhibitory effect of curcumin on proliferation of BGC-823 cells was dose dependent.Treatment with increasing dose of curcumin in the cells caused a dose-dependent apoptosis by flow cytometry analysis,such as 20mg/L curcumin pretreatment cause 48.3% cells apoptosis.Immunohistochem...
Objective: To investigate the anti-proliferative effect of Protobioside on the HepG2 cells and its mechanism.Method: The inhibitory effects of Protobioside on 3 kinds of human cancer cell line was measured by MTT assay.Apoptotic morphological changes was observed by Hoechst33528 staining technique...
2.8. MTT assay LLC cells in the logarithmic growth phase were harvested and adjusted to a cell density of 5 × 103 cells/mL post-trypsin digestion. These cells were then plated in 96-well plates at a volume of 100 μL per well. Following treatment with varying concentrations of FZQDP ext...
Selectivity of nrCap18 for cancer cells The selectivity of nrCap18 for cancer cells (MDA-MB 231) was investigated by comparing the cytotoxicity of this peptide by MTT assay after washing method in both the breast normal and cancerous cell lines. In the MDA-MB 231 cell line, the peptide ...
Methods Cardial fibroblasts(CFs) of neonatal Wistar rats were isolated and cultured,then was stimulated with AngⅡ.CFs proliferation was measured by thiazolyl blue(MTT)assay.mRNA expression of collagenⅠ,collagenⅢ,matrix metalloproteinase 2(MMP-2) was measured by reverse transcription-polymerase chain ...