The metabolic processes are tightly regulated and their alterations disturb function of stem cells. One of the indicators of metabolic status of cells is the ATP level. While the method of measuring the ATP levels has been known for many years, estimating ATP levels in small population of ...
Background Adenosine triphosphate (ATP) is the main energy carrier in living organisms, critical for metabolism and essential physiological processes. In humans, abnormal regulation of energy levels (ATP concentration) and power consumption (ATP consumption flux) in cells is associated with numerous dise...
They are based on various cell functions such as enzyme activity, cell membrane permeability, cell adherence, ATP produc- tion, co-enzyme production, and nucleotide uptake activity. Many have established methods such as Colony Formation method, Crystal Violet method, Tritium-Labeled Thymidine Uptake ...
A method for biomarker measurements in peripheral blood mononuclear cells isolated from anxious and depressed mice: β-arrestin 1 protein levels in depress... A limited number of biomarkers in the central and peripheral systems which are known may be useful for diagnosing major depressive disorders ...
It is one of the best-studied ATP-binding cassette transporters32 and is a homologue of the human gene MDR1 implicated in many drug resistant cancers33. Because PDR5 grants multidrug resistance, the maintenance of a bimodal population could be accompanied by fitness costs. To characterize the ...
, and there is provided a method for quantitatively determining an adenosine phosphate ester or a substance taking part in the ATP conversion reaction in high sensitivity and high accuracy using an inexpensive and simple measuring apparatus... M Seiji,S Tatsuya,E Naoki,... - EP 被引量: 22发...
These four methods were approved as alternate test procedures for measuring turbidity in drinking water in 2009 by the USEPA. The term “alternate test procedures” (ATP), is used as these methods use the same technique as an EPA-approved method (nephelometry) without creating...
Measuring mitochondrial bioenergetics in INS-1E insulinoma cellsPancreatic beta cells secrete insulin in response to raised blood glucose levels. This glucose-stimulated insulin secretion (GSIS) depends on mitochondrial function and is regulated by the efficiency with which oxidative metabolism is coupled ...
ATP (10 mM) Cell Signaling Technology Cat#9804 Protein Kinases Buffer (10×) Cell Signaling Technology Cat#9802 GST-YAP Homemade N/A 4% Paraformaldehyde Fisher Scientific Cat#50-259-98 Goat Serum Sigma-Aldrich Cat#G9023 ProLong® Gold Antifade Reagent with DAPI Invitrogen Cat#P36931 Poly-...
(upreg- ulation, 1.320 ± 0.228) and at 28 °C in the presence of SCC3193 (1.286 ± 0.159), with respect to cultures grown separately at 28 °C, and in the middle exponential phase cells in LB with respect to early stationary phase cells in the same medium (1.023...