将步骤3所得的感染细胞稀释至密度为1000cell/ml的单细胞悬液,并接种于96孔板的第一列,0.2ml/孔,从第一列吸取0.1ml单细胞悬液接种于第二列并加入0.1ml培养液混合,从第二列中吸取0.1ml接种于第三列并加入0.1ml培养液混合,一直到最后一列,再将最后一列的细胞放于显微镜下观察,挑选出只有单个细胞的孔并做好标...
Thus, a PBPK model with the incorporation of in vitro MDR1 and BCRP transporter function data and transporter protein expression levels has been developed. As such, it is crucial to generate MDR1 and BCRP substrate data with a high fidelity. In this study, two widely use human MDR1 cell ...
Key words: fetal tachyarrhythmia; breast cancer resistance protein; MDCKII-BCRP cell; MDCKII cell; transport mechanism; flecainide 乳腺癌耐药蛋白 (breast cancer resistance protein, BCRP 细胞模型系统地探讨这些治疗胎儿快速性心 [1] BCRP) 是 1998 年 Doyle 等 首次从乳腺癌细胞系 律失常的药物 ( ...
Thus, a PBPK model with the incorporation of in vitro MDR1 and BCRP transporter function data and transporter protein expression levels has been developed. As such, it is crucial to generate MDR1 and BCRP substrate data with a high fidelity. In this study, two widely use human MDR1 cell ...
Utilizing a Dual Human Transporter MDCKII-MDR1-BCRP Cell Line to Assess Efflux at the Blood Brain Barrier To facilitate the design of drugs readily able to cross the blood brain barrier (BBB), a Madin-Darby canine kidney (MDCK) cell line was established that over expresses both P-glycoprotei...
BCRP)等,MDCK—MDR1 细胞高表达目的蛋白P—gP, 可以排除这些干扰,专一地鉴定P—gP 在药物外排中 的作用,筛选它的底物及抑制剂,所以在许多研究中 用它的单细胞层来检测P—gP 底物和抑制剂的活性. Brayden 等用MDCK.MDR1 和MDCK—MRP 细胞 模型研究了阿弗菌素与P—gP 和MRP 的相互作用, 药学学报...
Keywords:MDCK—MDR1cell;P—glycoprotein;drugtransport;cellmod el 膜转运蛋白对药物的吸收,排泄及代谢,起着 至关重要的作用.它可以保护细胞免受外来毒性物 质侵害.将哺乳动物肿瘤细胞暴露在一种细胞毒类 药物中一段时间后,这些细胞可通过降低胞内药物
Placenta ABCG2 (BCRP) Transport Rabbit Developmental toxicity Pesticides 1. Introduction Pesticides are used in conventional agriculture to manage weeds, insects or fungi while non-agricultural pesticides (biocides) have a wider range of applications as disinfectants, preservatives, and in pest control....
Characterization of the transcellular transport properties of OATP1B3 substrates in new versions of double transfected MDCK II cells, OATP1B3/MDR1, OATP1B3/MRP2 and OATP1B3/BCRP The hepatobiliary transport is coordinated by both hepatic uptake and efflux processes. In the transport of organic anions,...
Liu Y Zeng S.Advances in the MDCK-MDR1 cell model and its applications to screen drug permeability [J][J].药学学报,2008,:43-564,559... Y Liu,S Zeng - 《Acta Pharmaceutica Sinica》 被引量: 35发表: 2008年 MDCKII/MDCKII-BCRP细胞模型用于治疗胎儿快速性心律失常系列药物的跨胎盘转运机制的...