The innate immune system initially recognizes RNA virus infection and evokes antiviral responses by producing type I interferons (IFNs). Toll-like receptors (TLRs) and cytoplasmic retinoic acid-inducible gene I (RIG-I)-like helicases (RLHs) are the two major receptor systems for detecting RNA ...
As CCDC50 could associate with RIG-I/MDA5/MAVS complex, we next tested how CCDC50 suppresses the activity of RLR. After recognition of viral RNA by RIG-I/MDA5, polyubiquitination and association of RIG-I/MDA5 with MAVS via their CARD domains are the important events for initiation of down...
37 你看看是不是你要的，我不确定，如果不是就抱歉了 RIG-1.jpg 虽然不是，不过thank you too ...
Here, we not only show that MDA5-mediated signaling is regulated by a dynamic balance between phosphorylation and dephosphorylation of its N-terminal CARDs but also identify the phosphatases PP1α and PP1γ as essential activators of both RIG-I and MDA5 signal transduction. Results The N-Termin...
Partial depletion and amino acid substitution analyses indicated that the F28A, V75A, and I96V mutations significantly compromised 2CCV-A6-induced MDA5/RIG-I depletion. Surprisingly, unlike V75A and I96V that interrupt the 2CCV-A6-MDA5/RIG-I interaction, 2CCV-A6 F28A remained competent ...
1.一种检测炎症性肌病特异性自身抗体MDA5的非放射标记免疫沉淀方法,其特征在于,包括以下步骤: 1)利用引物MDA5-F和MDA5-R从pENTER-MDA5质粒中扩增人MDA5的C末端; 2)回收纯化PCR片段克隆至pCMV-myc载体,构建含MDA5C末端以及N端带有Myc标签的质粒pCMV-myc-MDA5C,酶切鉴定确定pCMV-myc-MDA5C的插入子; 3)测序...
Comparative intermolecular interaction of RIG1 and MDA5 (human and buffalo) with dsRNA.Manvender, SinghBiswajit, BrahmaJitendra, MaharanaMahesh, Chandra PatraSushil, KumarPurusottam, MishraMegha, SainiBidhan, Chandra DeSourav, MahantyTirtha, Kumar Datta...
Ebrahim M, Mirzaei V, Bidaki R, et al. Are RIG-1 and MDA5 expressions associated with chronic HBV infection? Viral Immunol. 2015;28(9):504-508. doi:10.1089/vim.2015.0056Ebrahim M, Mirzaei V, Bidaki R, Shabani Z, Daneshvar H, Karimi-Googheri M, Khaleghinia M, Afrooz MR, Yousef...
In vitro studies suggest that both RIG-I and MDA5 detect RNA viruses and polyinosine-polycytidylic acid (poly(I:C)), a synthetic dsRNA analogue. Although a critical role for RIG-I in the recognition of several RNA viruses has been clarified, the functional role of MDA5 and the ...
RIG-I- and MDA5-initiated innate immunity linked with adaptive immunity accelerates beta-cell death in fulminant type 1 diabetes. Diabetes. 2011;60(3):884-9. doi: 10.2337/db10-0795. PubMed PMID: 21289206; PubMed Central PMCID: PMC3046849....