In this cell line, the PROTACs suppressed the residual expression of ERBB2/HER2, 3 and 4 that are essential for the proliferation of breast cancer cells and this cell line proved sensitive to HER2 inhibitors. In contrast, the effects of the PROTACs on the protein expression of MDA-MB-436 ...
在此基础上，研究蒙花苷对三阴性乳腺癌细胞株MAD-MB-231细胞AR的表达和细胞迁移的影响。1 材料和方法 1.1 试剂和仪器 MDA-MB-231细胞株购买于中国 科学院细胞库，目录号：TCHu227。蒙花苷（购自于上海源叶生物科技有限公司，批号：Z04A9L57508）。CCK-8试剂盒（日本同仁化学研究所，批号：TD826），反转录...
2 3SDF-1/CXCR4-PI3K信号通路抑制剂对MDA-MB-231细胞生长能力的影响:MTT实验结果显示:与对照组相比,AMD3100、LY294002及AMD3100+LY294002可明显抑制MDA-MB-231细胞的生长速率;并且24h、48h、72h、96小时后MDA-MB-231细胞的存活率逐渐降低(如图3A所示)。 软琼脂克隆形成实验结果显示:与对照组(如图3Ba所示)相...
4.血型抗原A 糖基转移酶通过诱导细胞发生ICD 而提高MDA-MB-231 的免疫原性。关键词: TNBC,血型抗原A 糖基转移酶,整合素,ADCC,CDC VII Abstract Study on anti-tumor effect of human blood group antigen A glycosyltransferase expression recombinant plasmid transfected human breast cancer cell MDA-MB-231 in ...
001,与阿霉素组比较;图2 DAPT及阿霉素对MDA-MB-231的抑制作用 2.2 DAPT及阿霉素对MDA-MB-231细胞周期分布的影响 DAPT组G1期细胞比例(43.61±0.02)%比对照组(37.98±0.01)%增加(P0.01,图3),联合用药组诱导细胞G1期阻滞(43.61±0.02)%比阿霉素组(57.65±0.03)%增加(P0.05)。 A:对照组;B:DAPT组;C:阿...
p65miRNA质粒明显下调MDA-MB-231细胞中 p65m R NA的表达水平,并显著抑制细胞中NF-KB的结合活性(P 胞G【 ,/G期细胞比例显著增加,S、 /M期细胞比例明显下降(P<0.05);Westernblot分析结果显示, 沉默p65基因后细胞中cyclinD1蛋白表达下调,p21蛋白表达增加。结论 ...
图3 木犀草素上调MDA-MB-231细胞OPCML mRNA和蛋白表达 Fig.3 Luteolin upregulates OPCML mRNA and protein expressions in MDA-MB-231 cells, *P < 0.05vs control group (0 μmol/L); A: Fold change of expression of OPCML mRNA; B: Expressions of OPCML proteins after luteolin treatment. 2.4 木犀草...
Furthermore, our data demonstrated for the first time that Gen inhibited the growth of MDA-MB-231 triple-negative breast cancer cells by inhibiting NF-κB activity via the Nocth-1 signaling pathway in a dose-dependent manner. We also found that Gen downregulated the expression of cyclin B1, ...
MDA-MB-231细胞增殖、周期、凋亡的影响。方法常规培养MDA-MB-231细胞 至对数期,随机分为对照组、DAPT组、阿霉素组及联合用药组。药物处理24h 后,CCK-8法检测细胞增殖情况,流式细胞仪检测细胞周期分布和凋亡率,Western Blot检测Notch-1,CyclinD1,PTEN和Bax蛋白的变化。结果DAPT对 MDA-MB-231细胞的抑制呈剂量依赖...
Therefore drug inhibiting the expression of HER2/neu can potently inhibit the cancer development. Hence in the current investigation we explored the potency of the synthesized curcumin coated gold nanoparticles against the HER2/neu expression in aggressive breast cancer cell type MDA-MB231/HER2 cell ...