骨肉瘤组织中MCM3AP-AS1的表达水平升高(P<0.05),miR-335的表达水平降低(P<0.05);转染si-MCM3AP-AS1可明显降低光密度值及Bcl-2蛋白水平,减少克隆形成数,提高凋亡率及Bax蛋白水平(P<0.05);双荧光素酶报告实验证实MCM3AP-AS1可靶向结合miR-335;转染miR-335 inhibitor可明显逆转干扰MCM3AP-AS1对U2OS细胞增殖及...
Long non-coding RNAs (lncRNAs) are a class of RNA molecules with transcripts longer than 200 nucleotides that have no protein-coding ability. MCM3AP-AS1, a novel lncRNA, is aberrantly expressed i
AS1在乳腺癌患者中的表达及其临床病理意义.方法:收集老年女性(年龄≥60岁)晚期乳腺癌患者肿瘤组织穿刺标本64例,应用qRT?PCR技术检测肿瘤组织中的LncRNA MCM3AP?AS1表达情况,免疫组化检测乳腺癌组织样本中的雌激素受体(estrogen receptor,ER),孕激素受体(progesterone receptor,PR),人表皮生长因子受体?2(human ...
In this work, MCM3AP-AS1, miR-138-5p and SIRT1 mRNA expression levels in OA and normal cartilage tissues were detected by qRT-PCR. Besides, chondrocyte cell lines, CHON-001 and ATDC5 induced by interleukin-1尾 (IL-1尾) were used to initiate the inflammatory response environment of OA....
It was found that MCM3AP-AS1 was declined in OA cartilage tissues, positively interrelated with SIRT1 expression while negatively correlated with miR-138-5p. MCM3AP-AS1 up-regulation enhanced the viability and migration of CHON-001 and ATDC5 cells while restraining the apoptosis and inflammatory ...
Minichromosome Maintenance Complex Component 3 Associated Protein Antisense 1 (MCM3AP-AS1) is an RNA gene located on 21q22.3. The sense transcript from this locus has dual roles in the pathogenesis of solid tumors and hematological malignancies. MCM3AP-AS1 has been shown to sequester miR-194-...
Expression levels of MCM3AP Antisense RNA 1 (MCM3AP-AS1), microRNA-126 (miR-126), and vascular endothelial growth factor (VEGF) in tissues and transfetced cells were measured by RT-qPCR. Cell transfections were performed to explore the interaction among MCM3AP-AS1, miR-126 and VEGF. ...
Knockdown of MCM3AP-AS1 or overexpression of miR-424-5p could reduce the absorbance(OD490) value of MDA-MB-231 cells, the number of cell migration and cell invasion, the expression levels of CyclinD1, N-cadherin and PSAT1 proteins(P<0.05), and i...
MCM3AP-AS1癌症结直肠癌预后目的:随着高通量测序技术的进步,大量研究发现长链非编码RNA(long non-coding RNA,lnc RNA)可作为一种新的诊断和预后生物标志物,并可能为临床提供新的治疗靶点.近期研究表明,MCM3AP反义RNA 1(MCM3AP antisense RNA 1,MCM3AP-AS1)在多种癌症中异常表达,参与肿瘤的发生,发展和转移....
LncRNA MCM3AP-AS1Dkk1骨转移预后目的:探讨长链非编码RNA(long noncoding RNA,LncRNA)MCM3AP?AS1在乳腺癌患者中的表达及其临床病理意义.方法:收集老年女性(年龄≥60岁)晚期乳腺癌患者肿瘤组织穿刺标本64例,应用qRT?PCR技术检测肿瘤组织中的LncRNA MCM3AP?AS1表达情况,免疫组化检测乳腺癌组织样本中的雌激素受体(...