In vivo assay revealed that MCM3AP-AS1 could hasten tumor growth in breast cancer by targeting CENPF. All results indicated that MCM3AP-AS1/miR-28-5p/CENPF axis accelerates breast cancer progression.doi:10.1016/j.biopha.2020.110289Qi Chen
Long non-coding RNAs (lncRNAs) are a class of RNA molecules with transcripts longer than 200 nucleotides that have no protein-coding ability. MCM3AP-AS1, a novel lncRNA, is aberrantly expressed i
据报道,长的非编码RNA MCM3AP反义RNA 1(MCM3AP-AS1)可以刺激多种类型肿瘤的增殖,迁移和侵袭。但是,尚不清楚MCM3AP-AS1在PC中的作用。使用实时定量PCR检测MCM3AP-AS1,microRNA miR-138-5p(miR-138-5p)和FOXK1水平。分析细胞增殖,迁移和侵袭。使用双重荧光素酶报告基因测定法来确认MCM3AP-AS1与miR-138-5p...
Knockdown of MCM3AP-AS1 or overexpression of miR-424-5p could reduce the absorbance(OD490) value of MDA-MB-231 cells, the number of cell migration and cell invasion, the expression levels of CyclinD1, N-cadherin and PSAT1 proteins(P<0.05), and i...
Although the fact that long non-coding RNA MCM3AP antisense RNA 1 (MCM3AP-AS1) is oncogenic in several cancers is well documented, very few researchers investigate its expression and function in prostate cancer.Paired prostate cancer samples were selected, and expressions of MCM3AP-AS1, miR-...
Differential expression of MCM3AP-AS1 in OA patients and healthy participants were analyzed by performing qPCR. A comparison of the expression level of MCM3AP-AS1 between OA group ( =70) and Control group (n=70) was performed by performing an unpaired t-test. PCR reactions were repeated 3...
骨肉瘤组织中MCM3AP-AS1的表达水平升高(P<0.05),miR-335的表达水平降低(P<0.05);转染si-MCM3AP-AS1可明显降低光密度值及Bcl-2蛋白水平,减少克隆形成数,提高凋亡率及Bax蛋白水平(P<0.05);双荧光素酶报告实验证实MCM3AP-AS1可靶向结合miR-335;转染miR-335 inhibitor可明显逆转干扰MCM3AP-AS1对U2OS细胞增殖及...
At first, MCM3AP‐AS1 expression was found to be elevated in lung cancer cells. Depletion of MCM3AP‐AS1 repressed cell proliferation, migration, and angiogenesis in lung cancer cells. YY1 was confirmed to mediate MCM3AP‐AS1 transcription in lung cancer cells. Moreover, the molecular ...
In this work, MCM3AP-AS1, miR-138-5p and SIRT1 mRNA expression levels in OA and normal cartilage tissues were detected by qRT-PCR. Besides, chondrocyte cell lines, CHON-001 and ATDC5 induced by interleukin-1尾 (IL-1尾) were used to initiate the inflammatory response environment of OA....
Functional rescue experiments showed that either inhibition of miR-149-5p nor ectopic expression of Notch1 dramatically weakened the biological impacts of MCM3AP-AS1 silencing. CONCLUSION :These finding demonstrated that SOX4-activated MCM3AP-AS1 aggravated OA progression by modulating autophagy and ECM...