This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits. ApplicationDilution Western Blotting 1:1000 Immunoprecipitation 1:50 Immunohistochemistry (Paraffin) 1:800 Immunofluorescence (Immunocytochemistry) 1:800 Chromatin IP 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), ...
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human MCM2 protein. Background The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for initiation and elongation of DNA replication. MCM2-7 bi...
Cell Signaling Technology product type : antibody product name : MCM2 Antibody catalog : 4007S quantity : 100 ul price : 235 USD clonality : polyclonal host : rabbit reactivity : human,mouse,rat application : western blot,immunohistochemistry,immunocytochemistry,immunoprecipitation ...
上海玉博生物科技有限公司 主要致力于“MCM2 Polyclonal Antibody BioVision-3758-100”的生产销售。多年的“MCM2 Polyclonal Antibody BioVision-3758-100”生产与销售的经验,与各行业新老用户建立了稳定的合作关系,我公司经营的产品名称深受广大用户信赖。欢迎来电咨询
AC133 is a type of antibody that is usually applied to isolate CSCs by testing a glycosylated epitope of CD133 on the cells [24]. Cell treatment CCSCs were transfected with 100 nM miR-195-5p/497–5p mimic or negative control (NC), 70 nM si-MCM2/p65 or NC, 100 nM pcDNA-...
al., 2013), (iii) usage of different experimental protocols or cell culture media, (iv) time window, in which synchronized cells are sorted/processed/analyzed, (v) the contribution of some differentiated cells, (vi) sensitivity of western blot assays, or (vii) quality of antibody reagents. ...
MCM4 and MCM5 followed by Western blot analysis. Membranes were first incubated with anti-O-GlcNAc antibody (RL2), stripped and then reprobed with anti-MCM antibodies.bMCF7 cells were synchronized in cell cycle by starvation (time point 0) then released in S phase (15H, 18H). Cell cycle...
HCT116 cells was treated with MP470 (0.1 µM, 24 h, AbMole), then the cells in the control and treated groups were incubated with 10 μM MG132 (Beyotime) for 6 h, immunoprecipitation was then performed using an anti-RUNX1 antibody (Proteintech, 25315-1-AP). The immunoprecipitated prot...
AC133 is a type of antibody that is usually ap- plied to isolate CSCs by testing a glycosylated epitope of CD133 on the cells [24]. Cell treatment CCSCs were transfected with 100 nM miR-195-5p/497– 5p mimic or negative control (NC), 70 nM si-MCM2/ p65 or NC, 100 nM pcDNA-...
PTEN and its associated proteins immunoprecipitated with a PTEN mouse monoclonal antibody (sc-7974) from cell extracts were subjected to western blotting using an MCM2 antibody generated in our laboratory and a PTEN rabbit polyclonal antibody (rabbit; CST no. 9559). In the input panel, MCM2 ...