为了解决这些问题,来自中东技术大学(Middle East Technical University)的 Pelin Toker、Hazal Ayten 等研究人员进行了深入研究,并在《Scientific Reports》期刊上发表了题为 “A reappraisal of cell cycle phase enrichment in synchronized estrogen receptor - positive cell models derived from breast adenocarcinomas”...
Trichostatin A and 5 Aza-2' deoxycytidine decrease estrogen receptor mRNA stability in ER positive MCF7 cells through modulation of HuR. Breast Cancer Res Treat 2008;111:15-25.Pryzbylkowski, P.; Obajimi, O.; Keen, J.C. Trichostatin A and 5 Aza-2' deoxycytidine decrease estrogen receptor ...
advancedbreastcancerthatishormonereceptorpositive/humanepidermalgrowthfactorreceptor2negative(HR+/HER2-).Chidamidehasbeenwidelyconcernedforitsadvantagesofcomfort,goodefficacy,strongtargeting,andfewadversereactions.However,drugresistanceusuallyoccursafterlong-termuse....
MCF-7 breast cancer cells are estrogen E2-sensitive cells expressing high levels ofERαtranscripts and very low levels ofERβ.MCF7 cells increase expression of estrogen receptor (ER) in the absence of estrogens. Response to E2 of MCF7 cells was demonstrated to be dependent on autocrine factor ...
Energy Balance Modulation Impacts Epigenetic Reprogramming, ERα and ERβ Expression, and Mammary Tumor Development in MMTV-neu Transgenic Mice The association between obesity and breast cancer risk and prognosis is well established in ER-positive disease but less clear in HER2-positive disease. Here,...
The gene fragment was then inserted into the vector pcDNA3.1 to construct the recombinant expression plasmid pcDNA3.1- HER2, which was identified by seguence analysis and transfected into ER-expressing MCF-7 cells Uia lipofectamine. The positive cell clones were obtained after G418 selection. The ...
A significant percentage (~30%) of estrogen receptor-α (ERα)-positive tumors become refractory to endocrine therapies; however, the mechanisms responsible for this resistance remain largely unknown. Chronic exposure to arsenic through foods and contaminated water has been linked to...
!"#2[ hich asidentifiedbyse uenceanalysisandtransfectedintoER_e pressingMCF_7cells$%&lipofectamine.The positivecellclones ereobtainedafterG418selection.TheMCF_7cellstransfected ith!"#2_cDNA asassayedby immunocytochemistry[flo cytometryandUesternblotting.>)-?"/-DNAse uencingresultssho edthat!"#2gene ...
(Fig.7a). Although we did not examine GR protein expression levels, it has been reported that ER/PR-positive and low-grade tumors express high levels of GR [29]. Moreover, only 3% of the 721 patient samples in our analysis had a mutation inNR3C1(data not shown). This finding suggests...
In ER-positive breast cancer cells, AR confers to be a good prognostic factor [43]. In ER-negative cases, the involvement of AR in promoting cell proliferation has also been observed [44]. Through stimulation with epinephrine, which acts via ARs, MCF7 cell viability and metabolism was ...