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The full-length human PAPP-A cDNA was optimized and synthesized, and the mature form (residues 1–1547) was sub-cloned into the pCAG vector with an IgG kappa signal peptide and an N-terminal maltose-binding protein (MBP)-His10 tag. Point mutations were introduced using a standard two-step...
This is a high enough frequency that most people cannot hear it, though many can sense it, mostly in the form of neck tension and mild headaches. If you’re being bothered by it, you can test for it: shut the computer down and see if it feels like a yoke has been lifted from off...
wasn’t really an expert in any way shape or form in those and I didn’t really have a passion for it and a lot of it started to be not related to what I was doing in my own life. So I had to start doing all this research into this dry information that I didn’t really care...