However, when the transporter is reconstituted in membrane bilayers, only the liganded form of MBP efficiently stimulates its activity. To investigate the mechanism of maltose stimulation, electron paramagnetic
2. Prediction of operons Multi-gene operons were predicted using our JPOP program [27, 28] for each genome. For genes arranged in tandem on the same strand and not predicted to be part of an operon by JPOP, we considered them to form an operon if their intergenic distances are shorter...
Expression of this cluster was restricted to Schwann cells and contained many genes related to myelin formation and neuronal transmission (GO:0007278, synaptic transmission, P < 0.00793; GO:0007399, nervous system development, P < 0.00793) including myelin basic protein (MBP), myelin ...
by treating unspliced intronic as a form of alternative splicing, BRIE2 allows us to quantify differential transcriptional kinetics between cell types, thus providing us with an efficient way to select biologically relevant features for RNA-velocity analyses. We show on a number of examples that thi...
[55,284,345,452]. Oligomers are seen as an intermediate structure, capable of inducing a conformational change in monomeric Tau which is then able to attach to the oligomeric structure. The latter then leads to the formation of stacked β-sheet-rich strands which grow to form insoluble paired...
maltose. A strong band corresponding to the size of MBP-I-DsqI can be seen in fractions 4 and 5. To test for DNA endonuclease activity, aliquots of fractions 1 ̶ 9 were incubated at 37 °C with linear target DNA containing the L2066 intron-lacking rDNA allele. After incubation and...
Kinase signaling pathways represent the most common form of reversible post-translational modifications that control many aspects of cellular function. Aberrant activation of protein kinases drive major hallmarks of malignancies, including alterations in cellular proliferation, survival, motility and metabolism,...
Bacterial expression and purification of recombinant proteins under homogeneous active form is often challenging. Fusion to highly soluble carrier proteins such as Maltose Binding Protein (MBP) often improves their folding and solubility, but self-association may still occur. For instance, HPV E6 oncopro...
High-grade gliomas (HGGs), which include grade III anaplastic astrocytomas and grade IV glioblastomas (GBMs), the deadliest form of brain tumor, are notoriously heterogeneous at the cellular level [1,2,3,4,5]. While it is well-established that transformed cells in HGG resemble glia [6, ...
It is possible that it can be involved in central carbon metabolism as well as be a component of the cell wall in the form of conjugates of mycolate, such as trehalose dimycolates and trehalose monomycolates [44],[46]. Many of the TCA intermediates such as succinic acid, citric acid ...