maltose. A strong band corresponding to the size of MBP-I-DsqI can be seen in fractions 4 and 5. To test for DNA endonuclease activity, aliquots of fractions 1 ̶ 9 were incubated at 37 °C with linear target DNA containing the L2066 intron-lacking rDNA allele. After incubation and...
Expression of this cluster was restricted to Schwann cells and contained many genes related to myelin formation and neuronal transmission (GO:0007278, synaptic transmission, P < 0.00793; GO:0007399, nervous system development, P < 0.00793) including myelin basic protein (MBP), myelin ...
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While no obvious differences in localization was observed using our Whrn antibody (data not shown) in any of these genotypes, we immunostained against 4.1B (Figure 5Aa,d–Da, d; red), Purkinje-specific Calbindin (Figure 5Ab–Db, green), and glial-specific myelin basic protein/MBP (Figure ...
Bacterial expression and purification of recombinant proteins under homogeneous active form is often challenging. Fusion to highly soluble carrier proteins such as Maltose Binding Protein (MBP) often improves their folding and solubility, but self-associ
While multiple tumors contained cells that express genes associated with more mature oligodendrocytes, the tumor PJ018 contained a well-defined subpopulation that strongly resembled myelinating oligodendrocytes with specific expression of multiple myelin genes including MBP, MOG, and MAG. Hence, HGGs ...
One of the essential processing events during pre-mRNA maturation is the post-transcriptional addition of a polyadenine [poly(A)] tail. The 3'-end poly(A) track protects mRNA from unregulated degradation, and indicates the integrity of mRNA through recog
form of AD [82,119,176,184], however there are also genetic and environmental risk factors [120,217,219] that contribute to familial or sporadic forms of AD, respectively. The pathophysiology of AD is complex and not fully understood as we will see through the course of this review ...
form a complex network that regulates plant flowering at the appropriate time based on changes in ambient conditions [8]. The four pathways are integrated by downstream target genes, includingLEAFY(LFY),FLOWERING LOCUS T(FT) andSUPPRESSOR OF CONSTANS1(SOC1), with their resulting outcomes conveyed...
Description of the Msm genome Mycobacterium smegmatis, a non-pathogenic, saprophytic, acid-fast, rod-shaped bacterium, has a GC rich genome of 7 Mbp, consisting of about 6938 genes. MC2 155, a reference strain of M. smegmatis is studied here, since it is widely used for experimental procedu...