Methods, Results & Conclusion NK cells from healthy donors were expanded for 14 days using irradiated K562 feeder cells displaying mbIL-21 and 4-1BBL (CSTX002) to derive mbIL-21 NK cells. These cells have minimal senescence despite robust proliferation with the feasibility to infuse multiple ...
We previously developed an ex vivo NK-cell expansion method based on K562 feeder cells modified to express membrane bound IL-21 (mbIL-21) and 4-1BB ligand, (FC21), which resulted in high numbers of hyperfunctional FC21-NK cells with enhanced cytotoxicity and cytokine production. Here we ...
另一个系统涉及 Epstein-Barr 病毒转化的淋巴母细胞饲养细胞,这导致 NK 细胞衰老前 2-4 周的强劲扩增[24]。为了解决衰老问题,K562 饲养细胞被设计为表达具有 4-1BB 配体的膜结合 IL-21 (mbIL-21),从而允许更长时间的 NK 细胞培养[21,22,25,26,27]。 为ACT 扩增 NK 细胞的其他方法包括使用永生化 NK ...