Methods, Results & Conclusion NK cells from healthy donors were expanded for 14 days using irradiated K562 feeder cells displaying mbIL-21 and 4-1BBL (CSTX002) to derive mbIL-21 NK cells. These cells have minimal senescence despite robust proliferation with the feasibility to infuse multiple ...
We previously developed an ex vivo NK-cell expansion method based on K562 feeder cells modified to express membrane bound IL-21 (mbIL-21) and 4-1BB ligand, (FC21), which resulted in high numbers of hyperfunctional FC21-NK cells with enhanced cytotoxicity and cytokine production. Here we ...
为了解决衰老问题,K562 饲养细胞被设计为表达具有 4-1BB 配体的膜结合 IL-21 (mbIL-21),从而允许更长时间的 NK 细胞培养[21,22,25,26,27]。 为ACT 扩增 NK 细胞的其他方法包括使用永生化 NK 细胞系,例如 NK-92 细胞。这种方法的一个主要挑战是必须在患者给药前对细胞进行辐照,这限制了这种治疗策略的功...
最近,使用辐照基因工程K562细胞(例如具有膜结合IL-21 (mbIL21) 和K562细胞系的基于K562的人工抗原呈递细胞)的方案报道了显着的高细胞毒性NK细胞扩增率修饰以表达本研究中使用的膜结合 IL-15(mbIL15)和4-1BB配体 (K562-mb15-41BBL) (7-9)。 小鼠胚胎饲养细胞通常用于长期培养程序和胚胎干细胞的集落再生,并且...