Our mutagenesis protocol avoids the problem of the inhibitory effect of the mutagen on seed germination and can be adapted to any callus induction/regeneration media with two modifications which are: the removal of agar and the addition of antioxidants during mutagenesis and rinsing. In addition, ca...
However, to date no studies of chemical mutagenesis in mature seed-derived rice calli in order to obtain mutant populations for TILLING have been reported. The aim of this work is to carry out a new TILLING strategy based on the production of a plant mutant population from EMS mutagenised ...
Diagram comparing callus mutagenesis and seed mutagenesis protocols in rice TILLING. (a) In the proposed rice TILLING protocol throughcallus mutagenesis,calli are induced, mutagenised, and the regenerated plants provide DNA for molecular screening of mutations.(b) In the basic TILLING method...