伴侣成对读取 正在读取配对……
Pevzner. De novo fragment assembly with short mate-paired reads: Does the read length matter? Genome Research, 19(2):336-346, 2009.Chaisson,M.J,Brinza,D,Pevzner,P.A.De novo fragment assembly with short mate-paired reads:does the read length matter?. Genome Research . 2009...
距离信息可以用来判定组装后成对 reads 间的序列是否准确,也可 用来帮助组装。这种测序方式可以用来解决基因组中的重复序列难题,被广泛采用。目前在 采用双端测序法时,454 平台建库最长(最长能达到 20k),Illumina 建库长度最短(小于 5k)。 由于 Solid 和Solexa 都是采用桥式扩增的方式,其本身自带 paired-end 测序...
single-read :单端测序(200-500bp) Paired-end :双末端测序(200-500bp)因为双末端测序,所以中间被测序列称为insert,insert打断了之后的片段就是reads。 Mate-pair:(2000-10000bp):用来生成确定基因中reads位置的短片段(end)。将一类长序列打断成特定大小(只要小于长片段长度即可),之后采用化学方式将其修复(环化等...
Two mate-paired libraries were separately constructed from human cancerous tissue to generate paired short reads (ditags) from restriction fragments digested with a 4-base restriction enzyme. A total of 3 Gb of paired reads (1.0脳 genome size) was collected, and 175 deletions were inferred by ...
For reads that don’t form a proper pair, or aren’t mapped at all, (almost) all bets are off. Pairs might be FF, RR or RF, and one might be mapped and the other not. Moreover, an unmapped read might have the 0×10 flag set, or not. ...
5. mate-paired 两端的序列形成”一对”,中间的距离叫插入长度(insert length, ins_length)。PE/MP 测序的结果除了序列本 身外还有中间的距离信息。距离信息可以用来判定组装后成对 reads 间的序列是否准确,也可 用来帮助组装。这种测序方式可以用来解决基因组中的重复序列难题,被广泛采用。。
Genome assembly scaffolding using information from paired-end/mate-pair libraries, long reads, and synteny to closely related species. - GitHub - lpryszcz/pyScaf: Genome assembly scaffolding using information from paired-end/mate-pair libraries, long re
An illumina paired-end and mate-pair short read simulator. This project attempts to model as many of the quirks that exist in Illumina data as possible. Some of these quirks include the potential for chimeric reads, and non-biotinylated fragment pull dow
A simple modification to the standard single-read DNA library preparation facilitates reading both the forward and reverse template strands of each cluster during one paired-end read. In addition to sequence information, both reads contain long range positional information, allowing for highly precise ...