We and others identified a long non-coding RNA associated with the MAPT gene, named MAPT antisense 1 (MAPT-AS1). We confirmed that MAPT-AS1 is expressed in neurons in human post mortem brain tissue. To study the role of MAPT-AS1 on MAPT expression regulation, ...
图2 乳腺癌细胞株T47D转染长非编码MAPT-AS1效率验证及其过表达和干扰的相对表达量Fig.2 Efficiency verification and overexpression and interference relative expression of breast cancer cell line T47D transfected with lncRNA-MAPT-AS1 图3 长非编码MAPT-AS1共表达基因表达水平Fig.3 lncRNA-MAPT-AS1 co-exp...
目的 探讨长链非编码(Lnc)RNA锌指蛋白ZBTB20反义RNA1(ZBTB20-AS1)靶向miR-132-3p/微管相关蛋白tau(MAPT)轴对阿尔茨海默病(AD)发生发展的影响.方法 采用Aβ25～35(20μmol/L)处理SK-N-SH细胞构建AD细胞模型.将si-con、si-ZBTB20-AS1、miR-con、miR-132-3p mimics、si-MAPT分别转染SK-N-SH细...
In addition, the level of MAPT-AS1 was correlated with the expression of MAPT, and MAPT was associated with survival time in breast cancer. Our study suggests that MAPT-AS1 may play a role and be a potential survival predictive biomarker in breast cancer....
【结果】荧光显微镜观察及RT-qPCR验证,成功构建长非编码MAPT-AS1过表达及干扰稳定转染乳腺癌细胞株T47D,并筛选出干扰效率最高的长非编码MAPT-AS1干扰片段shRNA3。验证其共表达基因得到MAPT、MAPT-IT1和NXNL2在转染了干扰片段shRNA3后表达量降低,与长非编码MAPT-AS1的表达趋势一致。【结论】成功构建长非编码MAP...
A GENE THERAPY APPROACH FOR REDUCTION OF TAU BY TAU LONG NON-CODING RNA GENE (MAPT-AS1) NATURAL ANTISENSE TRANSCRIPTdoi:10.1016/j.jalz.2018.07.086de Silva, RohanZareba-Paslawska, JustynaAlmeida, FilipaSvenningsson, PerWarner, TomSimone, Roberto...
Effect of MAPT-AS1 over- and knock-down expression on MAPT expression in HEK293 and SK-N-MC cells.doi:10.1371/JOURNAL.PONE.0157924.G002G. CouplandKirstenS. WoojinM. GlendaH. MarianneD. CarolB. JohnPLOS ONE
Insights into the noncoding RNA-encoded peptides.doi:10.1159/000491767The regulatory role of long non-coding RNAs (lncRNAs) in various biological functions has been demonstrated. However, their role in fat deposition and lipi...