Here, we introduce magnetic bead-assisted parallel single-cell gene expression sequencing (MAPS-seq), a microwell-based method that pools samples before the reverse transcription step, increasing the ease of sample preparation and reducing reagent waste. Moreover, because this method uses universal ...
宏基因组绘图采样测序（MaPS-seq），实现在微米级分辨率下表征微生物组的空间结构；将完整的微生物组样品固定在凝胶基质中并冷冻破碎成颗粒，然后通过基于液滴的包封，条形 码化16S rRNA扩增和深度测序鉴定颗粒中相邻的微生物类群；对小鼠肠道的三个区域的分析揭示了微生物分布异质性，以及特定分类群之间的正和负相关联...
图b:由混匀的粪便和大肠杆菌的制备的模拟群落簇进行MaPS-seq测序。散点图展示的是由339个簇通过MaPS-seq测序得到的序列,这些序列属于粪便或者大肠杆菌中的OTU。混合的序列很少,表明了每个cluster可以很好地区分测序。 图c:通过普通16S对混匀的粪便样品测序和基于MaPS-seq对每个粪便样品的cluster的测序结果做皮尔森相关。
The MaPS-seq processing script is called from the command line and takes the following arguments. In addition, a file named param must be included in the same directory which includes the structure of the barcode utilized (anchor sequences and barcode sequences). The script calls barcodes, ren...
et al. Trans-Seq maps a selective mammalian retinotectal synapse instructed by Nephronectin. Nat Neurosci 25, 659–674 (2022). https://doi.org/10.1038/s41593-022-01068-8 Download citation Received24 June 2021 Accepted30 March 2022 Published06 May 2022 Issue DateMay 2022 DOIhttps://doi.org...
Human fetal germ cells (FGCs) are precursors to sperm and eggs and are crucial for maintenance of the species. However, the developmental trajectories and heterogeneity of human FGCs remain largely unknown. Here we performed single-cell RNA-seq analysis of over 2,000 FGCs and their gonadal niche...
TT-seq maps the human transient transcriptome Pervasive transcription of the genome produces both stable and transient RNAs. We developed transient transcriptome sequencing (TT-seq), a protocol that un... Björn,Schwalb,Margaux,... - 《Science》 被引量: 75发表: 2016年 A Strand-Specific RNA–...
Bosniya və Herseqovina - Bosniya - Bosniya və Herseqovina kart. Bosniya və Herseqovina - Bosniya - Bosniya və Herseqovina xəritədə. Kart Bosniya və Herseqovina - Bosniya - Bosniya və Herseqovina Cənubi Avropa - Avropa. Kart
About Sequim, WA The Valley of the Dungenesslies at the northern endof the Olympic Peninsula, in the extreme northwest corner of the United States. It spreads in an alluvial fan from the Dungeness River gorge to the scalloped beaches of the Strait of Juan de Fuca. ...
今天的笔记是关于另一种RNA测序的方法,称为TT-seq。其实这些测序方法在几年前被人们所使用了,但是因为自己并不了解这些知识,所以要慢慢的补起来。 文献题目:TT-seq maps the human transient transcriptome 发表时间:2016 杂志:Science 摘要 基因组的普遍转录产生稳定的和不稳定的RNA(瞬时的)。作者开发了一种瞬时转...