宏基因组绘图采样测序（MaPS-seq），实现在微米级分辨率下表征微生物组的空间结构；将完整的微生物组样品固定在凝胶基质中并冷冻破碎成颗粒，然后通过基于液滴的包封，条形 码化16S rRNA扩增和深度测序鉴定颗粒中相邻的微生物类群；对小鼠肠道的三个区域的分析揭示了微生物分布异质性，以及特定分类群之间的正和负相关联...
图b:由混匀的粪便和大肠杆菌的制备的模拟群落簇进行MaPS-seq测序。散点图展示的是由339个簇通过MaPS-seq测序得到的序列,这些序列属于粪便或者大肠杆菌中的OTU。混合的序列很少,表明了每个cluster可以很好地区分测序。 图c:通过普通16S对混匀的粪便样品测序和基于MaPS-seq对每个粪便样品的cluster的测序结果做皮尔森相关。
A comprehensive step-by-step protocol to performing a MaPS-seq experiment can be foundhere. Microfluidic chip design The microfluidic device design can be foundhere. We ordered the wafer from FlowJEM with a feature height of ~40 μm.
et al. Trans-Seq maps a selective mammalian retinotectal synapse instructed by Nephronectin. Nat Neurosci 25, 659–674 (2022). https://doi.org/10.1038/s41593-022-01068-8 Download citation Received24 June 2021 Accepted30 March 2022 Published06 May 2022 Issue DateMay 2022 DOIhttps://doi.org...
In the current round of SEQ regional planning, the SEQ 2021 GIS Unit provides a service, which facilitates, coordinates and leads the use of spatial information to increase SEQ 2021 stakeholder understanding of the region's issues and, as a result, build the capacity and confidence of ...
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et al. Single-cell ATAC-seq maps the comprehensive and dynamic chromatin accessibility landscape of CAR-T cell dysfunction. Leukemia 36, 2656–2668 (2022). https://doi.org/10.1038/s41375-022-01676-0 Download citation Received18 February 2022 Revised28 July 2022 Accepted01 August 2022 Published...
BMC Genomics (2017) 18:125 DOI 10.1186/s12864-017-3511-2 METHODOLOGY ARTICLE Open Access Exome QTL-seq maps monogenic locus and QTLs in barley Hiroshi Hisano1†, Kazuki Sakamoto1†, Hiroki Takagi2, Ryohei Terauchi2 and Kazuhiro Sato1* Abstract Background: QTL-seq, in combination with ...
The BGI-research team wants to use the Stereo-Seq technology to create more maps. Indeed, they posteda preprintin March of this year describing a comprehensive single-cell spatial transcriptome map of monkey brains. They used this technology to analyze 130 slides in about one week’s time, Ch...
宏基因组图采样测序(MaPS-seq),它是一种不依赖于培养的方法,实现于以微米级分辨率表征微生物组的空间组织; 将完整的微生物组样品固定在凝胶基质中并冷冻破碎成颗粒。然后通过基于液滴的包封,条形码化16S rRNA扩增和深度测序鉴定颗粒中相邻的微生物类群。