Here, we introduce magnetic bead-assisted parallel single-cell gene expression sequencing (MAPS-seq), a microwell-based method that pools samples before the reverse transcription step, increasing the ease of sample preparation and reducing reagent waste. Moreover, because this method uses universal ...
宏基因组绘图采样测序（MaPS-seq），实现在微米级分辨率下表征微生物组的空间结构；将完整的微生物组样品固定在凝胶基质中并冷冻破碎成颗粒，然后通过基于液滴的包封，条形 码化16S rRNA扩增和深度测序鉴定颗粒中相邻的微生物类群；对小鼠肠道的三个区域的分析揭示了微生物分布异质性，以及特定分类群之间的正和负相关联...
图a:基于MaPS-seq测序小鼠末端结肠的簇的平均直径为30微米;热图展示的是基于MaPS-seq测序原始相对丰度数据;列代表1406个簇,行代表来自同于个样品的两个技术重复中含量丰富的和在簇中普遍存在的OTU。(相对丰度大于2%,在超过10%的簇中出现的24个共有OTU,总数为246个),热图阴影代表每个OTU的相对丰度。OTU在热图上的...
MaPS-seq: Metagenomic Plot-sampling by sequencing Code, protocols and additional information from the paper "Spatial metagenomic characterization of microbial biogeography in the gut" Sheth RU, Li M, Jiang W, Sims PA, Leong KW, Wang HH. Nature Biotechnology, 2019 The full paper and supplemental ...
We used Trans-Seq to classify SC neuron types innervated by genetically defined RGC types and predicted a neuronal pair from αRGCs to Nephronectin-positive wide-field neurons (NPWFs). We validated this connection using genetic labeling, electrophysiology and retrograde tracing. We then used ...
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Human fetal germ cells (FGCs) are precursors to sperm and eggs and are crucial for maintenance of the species. However, the developmental trajectories and heterogeneity of human FGCs remain largely unknown. Here we performed single-cell RNA-seq analysis of over 2,000 FGCs and their gonadal niche...
Based on our single-cell RNA-seq data, one of the NOTCH ligands, DLL3, was expressed not only in FGCs but also in a fraction of gonadal somatic cells, providing a foundation for NOTCH signaling interaction between different gonadal somatic cells. In addition, by GSEA, we found that human ...
MAPS (Model-based Analysis of PLAC-Seq data) pipeline is a a set of multiple scripts used to analyze PLAC-Seq and HiChIP data. MAPS pipeline contains multiple scripts. The run_pipeline.sh is a prototypes of a shell script containing the locations of files needed to run MAPS is entered as...