研究结果表明，G6PD可能通过增加基质金属蛋白酶2（MMP2）mRNA和蛋白质在体内外的表达，促进ccRCC细胞侵袭能力。此外，通过RT-qPCR和蛋白印迹的方式，对20种ccRCC肿瘤标本和相匹配的邻近正常组织进行调查研究，证实了G6PD与MMP2表达呈正相关关系。此外，G6PD在ccRCC细胞中促进了活性氧（ROS）生成并激活MAPK信号通路。 ROS...
ROS显著促进MAPK信号通路的激活,进而导致ccRCC细胞中MMP2的过表达。上述研究内容表明,G6PD通过ROS-MAPK轴途径,促使MMP2的过表达,从而增强ccRCC细胞的侵袭能力。 SP600125 (Abmole, M2076,纯度:99.13%) ,是一种高特异性的,有效的MEK1/2抑制剂,IC50为0.92 nM/1.8 nM,对c-Raf, B-Raf, ERK1/2没有抑制活性。
结果与对照组比较,10,20,40 mg/L能够以剂量依赖性的方式降低细胞侵袭数目[(324.59±52.31)比(242.33±34.32),(175.56±25.42),(121.23±21.23),F=18.33,P<0.01],MMP2[(1.00±0.00)比(0.67±0.09),(0.40±0.06),(0.19±0.03),F=23.82,P<0.01]及MMP9[(1.00±0.00)比(0.74±0.13),(0.54±0.07),(0.34...
ccRCC MAPK/MMP2 pathway 1. Introduction Renal cell carcinoma (RCC) is one of the most lethal urological malignancies in the urological system, arising from the proximal tubular epithelial cells of the kidney. The prevalence of RCC has been increasing annually, ranking ninth in men and fourteenth...
LPS triggered inflammatory level, cell migration, and matrix metalloproteinase (MMP) 2/9 production, which was reduced in ZO-treated cultures. TAK1 inhibition by ZO also decreased activation of MAPKs pathway, indicating that ZO-mediated alleviation of neuroinflammation is likely modulated via TAK1/...
Employing MMP2-activation of genetic editing within the cell membrane and US-responsive BTO, a nanoplatform is created for both immune stimulation and targeted PD-L1 blockage, offering a secure and strong means of improving the immune system's action against tumor cells.For severe adolescent ...
经2周治疗后茵陈蒿汤能显著降低DMN诱导的大鼠血清肝功能水平,抑制组织炎细胞的浸润与坏死,胶原沉积,并下调了 肝组织IL-1 b,Cd68,Tnfrsf14,Tn-frsf9,Fas,Cd14,Ctgf,Col1 a2,Igfals,Igf1,Igfbp1,Mmp12,Mmp2,Mmp23,Ccl21,Prkcb 基因的表达,抑制了MAPK通路的活化.通过全基因芯片的分析,在茵陈蒿汤干预治疗后...
Up-regulation of MMPs is one of the processes by which p38MAPK promotes cell migration and invasion in tumors. Past reports have shown high MMP-2/9 activity in HTB9 cells, while in HTB5 cells MMP-9 activity in a basal state was low. Additionally, it has been observed that p38MAPK sign...
抑制因子也可极大程度抑制PMA 诱导的MMP 29分泌,但对uPA 无影响。细胞外基质金属蛋白酶诱导剂(EMMPRIN )可通过p38MAP K 诱导肺癌旁间质纤维母细胞MMP \|1表达,与ER K1/2和J N K/SAP K 无关。 研究显示口腔癌细胞的侵袭是通过转录因子AP 21依赖性机制促进MMPs 表达的;AP 21的活性和表达部分由ER K1/...