DNA Restriction EnzymesChromosome MappingPlasmidsMolecular WeightNostoc 7524 contains three different plasmids of molecular weight, 4, 8, and 28 Mdal. The smallest plasmid, designated pDU1, because of its size and ease of isolation, may prove to be useful as a cloning vector. Plasmid pDU1 was ...
such as the origin of replication, genes of interest, restriction enzyme sites, and primer binding sites, in addition to the plasmid name and length. Plasmid maps are important for planningcloning experiments, as well as for publication and collaboration...
COLICIN E1 plasmid (colE1) is a closed circular DNA molecule with a molecular weight of 4.2 × 106(ref. 1). ColE1 DNA has extensively been used as a molecular vehicle for cloning and amplification of DNA in genetic engineering2,3. In order to expand such investigations, it is useful to...
Plasmids can mediate horizontal gene transfer of antibiotic resistance, virulence genes, and other adaptive factors across bacterial populations. Here, we analyze genomic composition and pairwise sequence identity for over 10,000 reference plasmids to obtain a global map of the prokaryotic plasmidome. ...
Global insights into cellular organization and genome function require comprehensive understanding of the interactome networks that mediate genotype–phenotype relationships1,2. Here we present a human ‘all-by-all’ reference interactome map of human binary protein interactions, or ‘HuRI’. With approxim...
pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long. 来自 掌桥科研 喜欢 0 阅读量: 79 作者: Sutcliffe J.Gregor 摘要: I have derived a complete restriction map of pBR322 from the total nucleotide sequence of the plasmid. Most ...
Children with trisomy of chromosome 21 (Ts21), known as Down syndrome, have a 150-fold higher risk of developing myeloid leukaemia in the first 5 years of life than the general population1,2. The current leukaemogenesis model in Down syndrome proposes that specific somatic mutations in the ...
摘要: The cleavage sites of the restriction nucleases HpaI, HpaII, HindII, HindIII, and PstI have been mapped on the DNA of the bacteriophage PM2. This map has been used for the localization of two strong binding sites of Escherichia coli RNA polymerase on PM2 DNA....
Following overnight growth of glycerol stocks arrayed in 384-well format [59], plasmid DNA was extracted using a standard lysis and neutralization procedure followed by an alcohol precipitation. Sequencing was performed on an ABI 3730 sequencer using BigDye™ Terminator V3.1 (ABI) cycle sequencing...
The preservation of genetic information is a fundamental cellular process that represents a formidable challenge given that, in each cell and on a daily basis, the DNA polymer is the subject of a wide variety of chemical and physical alterations. These alterations, known collectively as DNA damage...