Methods: Multiple tissue clearing methods were applied to intact virgin and lactating mammary glands, namely 3D imaging of solvent-cleared organs, see deep brain (seeDB), clear unobstructed brain imaging cocktails (CUBIC) and passive clarity technique. Using confocal, two-photon and light sheet ...
Mammary glands are unique to mammals, with the specific function of synthesizing, secreting, and delivering milk to the newborn. Given this function, it is only during a pregnancy/lactation cycle that the gland reaches a mature developmental state via hormonal influences at the cellular level that...
Elucidation of the bovine mammary gland transcriptome by RNA-seq is essential for identifying candidate genes that contribute to milk composition traits in dairy cattle. Results We used massive, parallel, high-throughput, RNA-seq to generate the bovine transcriptome from the mammary glands of four ...
Heid HW, Winter S, Bruder G, Keenan TW, Jarasch E-D (1983) Butyrophilin, an apical plasma membrane-associated glycoprotein characteristic of lactating mammary glands of diverse species. Biochim Biophys Acta 728: 228–238 Google Scholar Helminen HJ, Ericsson JLE (1968) Studies on mammary gland...
Multiple tissue clearing methods were applied to intact virgin and lactating mammary glands, namely 3D imaging of solvent-cleared organs, see deep brain (seeDB), clear unobstructed brain imaging cocktails (CUBIC) and passive clarity technique. Using confocal, two-photon and light sheet microscopy, ...
Consequently, there is a compelling need for the development of an effective in vitro model to comprehensively study the lactation biology of the mammary gland in ruminants. The adoption of 3D-cultured models has emerged as a valuable approach to modeling the function of mammary glands in dairy ...
Endocrinology 148: 5943 – 5954 Mammary microcalcifications: beyond the mammogram RF Cox et al 537 Virk SS, Kirk CJ, Shears SB (1985) Ca2+ transport and Ca2+-dependent ATP hydrolysis by Golgi vesicles from lactating rat mammary glands. Biochem J 226: 741 – 748 Wang X, Chao L, Chen L...
2. Estrous cycle monitoring of adult FVB/N mice and carmine staining of pubertal glands. Supplementary Fig. 3. FACS strategy. Supplementary Fig. 4. Estrous cycle monitoring of adult FVB/N mice for whole mount carmine staining. Supplementary Fig. 5. Whole mammary glands in estrus and diestrus ...
With this new gene delivery system, genetically manipulated mammary glands were successfully reconstituted even though the vector size was > 200 kb and even in the presence of DNA elements such as promoters and transcription termination sequences, which are major obstacles to viral vector packagin...
There have been numerous efforts to analyse DNA methylation patterns of stem/progenitor cells and differentiated luminal and myoepithelial cells in mice and humans [26,27,28,29,30]. Stem/progenitor cells isolated from human mammary glands are hypomethylated compared with differentiated luminal and myo...