Although several cell culture systems have been developed to investigate the function of the mammary gland in dairy livestock, they have potential limitations, such as the loss of alveolar structure or genetic and phenotypic differences from their native
Model for hormonal regulation of claudin-3 and claudin-4 in the lactating mammary gland. Data from cultures of mouse mammary epithelial cells show that claudin-3 and claudin-4 respond to prolactin and glucocorticoids, major regulators of secretory activation in a manner similar to casein and lipid...
Proliferating cells (BrDU labeled, green) and PR positive cells (red) are generally not coincident. Note that the number of proliferating cells is reduced in the absence of PRL. Scale bar, 50 μm.Source: (A) Used by permission from Ref. 93. (B) Original figure from Ref. 64. The ...
Organization of the epithelium in lactating cow mammary gland. A Section of mammary tissue analyzed by indirect immunofluorescence and labeled for the keratins KRT14 and KRT7, and type I collagen for the extracellular matrix. Nuclei were counterstained with DAPI. B Schematic representation of the ep...
英文名称: MouseMammaryGland:NormalMammaryGlandEpithelialCells 货号: CS-2123X 保存条件: 低温冷藏 本公司提供的细胞都是现货供应,详细小鼠乳腺上皮细胞 MouseMammaryGland:NormalMammaryGlandEpithelialCells说明书! 小鼠乳腺上皮细胞 MouseMammaryGland:NormalMammaryGlandEpithelialCells 产品名称 英文名称 价格 小鼠乳腺...
(a) mammary gland reconstitution. (b) MaSCs upon oncogenic transformation as cell-of-origin model (albeit unconfirmed). In both cases, fibroblasts are co-injected with human MECs for humanization. CD49f (α6-integrin).9,10 It was the first time to provide functional evidence that a single...
At present, few details exist about how these processes are regulated within the mammary gland. However, recent advances in our understanding of the structural and molecular biology of membrane systems and cellular lipid trafficking provide insights into the mechanisms underlying the regulation and ...
9 Exploration of the dynamics of the stem cell fate transition using mini gland. a, Bright field image of d6 organoids. b, The workflow to analyze the fate of labeled cells after 1d tracing. c, FACS dot plots of luminal cells and basal cells gated within the tdTomato positive and GFP...
Cells were metabolically labeled with 10 μmol/L bromodeoxyuridine for four hours, trypsinized, and fixed with ice-cold 70% ethanol. Nuclei were isolated and stained with propidium iodide and FITC-conjugated anti-bromodeoxyuridine antibodies (Becton Dickinson, San Jose, CA, USA) and analyzed by fl...
Fig. 1.Schematic representation of the nucleosomal organization of the mouse mammary tumor virus (MMTV) LTR. When stably introduced into cells, the MMTV promoter organizes into a phased array of nucleosomes, labeled A to F. High-resolution mapping of the nucleosome boundaries identified a ser...