Development of the mammary gland during puberty, pregnancy, and lactation is controlled by steroid and peptide hormones and growth factors. To determine th... S Neuenschwander,A Schwartz,TL Wood,... - 《Journal of Clinical Investigation》 被引量: 632发表: 1996年 Rapidly labeled, polydisperse RN...
Although several cell culture systems have been developed to investigate the function of the mammary gland in dairy livestock, they have potential limitations, such as the loss of alveolar structure or genetic and phenotypic differences from their native
Proliferating cells (BrDU labeled, green) and PR positive cells (red) are generally not coincident. Note that the number of proliferating cells is reduced in the absence of PRL. Scale bar, 50 μm.Source: (A) Used by permission from Ref. 93. (B) Original figure from Ref. 64. The ...
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9 Exploration of the dynamics of the stem cell fate transition using mini gland. a, Bright field image of d6 organoids. b, The workflow to analyze the fate of labeled cells after 1d tracing. c, FACS dot plots of luminal cells and basal cells gated within the tdTomato positive and GFP...
The characteristics of pregnant rat mammary gland progesteronee receptors have been studied by the use of two synthetic probes, [3H]R5020 (3H-labeled 17 alpha, 21-dimethyl-19-nor-pregn-4,9-diene-3,20-dione) amd [3H]ORG 2058 (3H-labeled 16 alpha-ethyl-21-hydroxy-19-nor-pregn-4-ene-...
“parity-labeled” or “parity-amplified” as they are present before pregnancy [81]. The description of this population pioneered inducible lineage tracing in the mammary gland [79]. Here, transgenic mice-expressing Cre recombinase under control of the mammary (and pregnancy)-specific promoter for...
Fig. 1.Schematic representation of the nucleosomal organization of the mouse mammary tumor virus (MMTV) LTR. When stably introduced into cells, the MMTV promoter organizes into a phased array of nucleosomes, labeled A to F. High-resolution mapping of the nucleosome boundaries identified a s...
The histological appearance of the tumor was highly similar to that of the mouse mammary tumor virus-PyMT transgenic mouse model. Conclusions With this system, gene transduction in the mammary gland can be easily and quickly achieved, and gene expression can be controlled by Dox administration. ...
Macrophages serve multiple functions including immune regulation, morphogenesis, tissue homeostasis and healing reactions. The current paradigm holds that mammary gland macrophages first arise postnatally during the prepubertal period from the bone marrow-derived monocytes. Here we delineate the origins of ti...