Injured 尾-secretase 1 (BACE1) knockout mice have enhanced macrophage recruitment and debris clearance, which may be due to BACE1 activity in macrophages or the hypomyelination observed in BACE1 knockout mice. To assess if BACE1 expression by macrophages mediates enhanced macrophage recruitment we ...
To explore the role of YAP in the regulation of SIRT1 expression and HMGB1 acetylation following lactate treatment, we isolated peritoneal macrophages from both wild type (WT) and macrophage-specific YAP knockout (YAPfl/fl; Lyz2-Cre+) mice (Figure S6A) and examined SIRT1 expression and HMGB1...
Using macrophage-specific Notch1 knockout mice (NOTCH1螖Myelo) in conjunction with AAV-F4/80-mediated NICD1 overexpression, we investigated the impact of Notch1 on septic cardiac injury. LPS-stimulated bone marrow-derived macrophages (BMDMs) were analyzed by flow cytometry and ELISA to assess ...
Such observations have been confirmed in studies conducted with global SR-BI knockout mice, whereby these animals display an increase in cardiovascular disease despite elevated plasma levels of HDL-associated cholesterol35. Endothelial SR-BI mediates the transport of both HDL and LDL to the sub...
Hypoxia-inducible factor-α knockout mice GBM: Glioblastoma TIE: Tyrosine kinase with immunoglobulin-like and EGF-like domains mAB: Monoclonal antibody i.p.: Intraperitoneal Jmjd3: Jumonji domain containing-3 H3k27: Histone 3 Lys27 ROS: Reactive oxygen species SHIP: Src-homology 2-...
mice [64]. Quaking (QKI) is an RNA-binding protein that can exert intestinal protective effects, and QKI-knockout mice showed elevated susceptibility to DSS and an increased proportion of M1 macrophages in the colon, with in vitro co-culture experiments suggesting that QKI deficiency could ...
The number of small peritoneal macrophages (SPM) in the peritoneal exudate of CCR2 knockout (KO) mice was notably decreased, while the number of LPMs was unaffected. This observation suggested that the majority of SPMs originate from the inflammatory monocyte population [26]. The distribution and...
Interestingly, evidence from knock-out (KO) mouse studies suggest that the steady state maintenance of myeloid lineage cells depend more critically on G-CSF, M-CSF and Fms like tyrosine kinase-3 ligand (Flt3L) while GM-CSF is largely dispensable for this purpose [1]. GM-CSF KO...
specific macrophage populations. For example, administration of a peptide sequence unique to tissue remodeling/fibrotic (M2) macrophages identified by subtractive phage biopanning and fused to the KLA proapoptotic peptide [286] decreased mortality in CT-26 tumor-bearing mice by inducing a decreased ...
Moreover, the M2-type macrophage polarization and liver fibrosis were examined in hepatocyte-specific PGC-1α knockout mice and AAV8-mediated PGC-1α over-expressing mice following liver I/R. The down-regulated PGC-1α expression by I/R was negatively correlated with IL-6 levels in the ...