Regulation of macrophage gene expression following invasion by Mycobacterium tuberculosis.Mycobacterium tuberculosisMacrophage infectionDifferential display RT-PCRIntroduction: Mycobacteria are intracellular pathogens which survive and grow in host macrophages. M. tuberculosis bacilli enter the macrophage via binding ...
we found that SARS-CoV-2 induced gene expression of PTX3 in respiratory tract epithelial cells. Using bulk RNA-seq and bioinformatic analysis at single-cell level, we found that PTX3 was selectively expressed by monocytes among circulating leukocytes...
RT-qPCR was carried out to determine specific gene expression using the SYBR Green Real-time PCR Master Mix (ATP-111, TOYOBO, Osaka, Japan) on StepOne Plus (Thermo Fisher Scientific, Waltham, MA, USA) by the manufacturer. The quantification of target genes was identified by comparing Ct ...
the activation of STAT1 and STAT6, respectively. The PI3K/Akt pathway is activated in M2 macrophages and promotes M2-associated gene expression through PPAR-γ. The MAPK pathway, including ERK, JNK, and p38, is activated in M1 macrophages and promotes the expression of pro-inflammatory cytokines...
PCR amplifications were performed in a total volume of 20 μL, containing 20× TaqMan Expression Assay primers, 2× TaqMan Universal Master Mix (Applied Biosystems, TaqMan® Gene Expression Systems), and cDNA template. Each PCR amplification was performed in duplicate wells in a StepOne™ ...
Leishmania qRT-PCR: Quantitative real time PCR GEO: Gene expression omnibus MIAME: Minimum information about a microarray experiments KEGG: Kyoto Encyclopedia of genes and genomes.References Buates S, Matlashewski G: General suppression of macrophage gene expression during Leishmania donovani infe...
The basal level of expression of M-CSF/CSF-1R was examined in retinal microglial cells in vitro. Immunofluorescence, real-time PCR, immunoprecipitation, and Western blot analyses revealed the up-regulation of CSF-1R in GA-treated microglial cells. We also detected increased expression and release ...
trium tuberculosisisocitrate lyase mutants have elevated antibiotic-induced ROS levels56, we also deletedC. glabrataisocitrate lyase geneICL1(CAGL0L09273g). All deletion mutants grew normally in YPD broth (Fig.5a). Consistent with their compromised ROS detoxification activities, most of the mutants ...
Transduced cells were selected with 2 μg/ml puromycin (Sigma, St. Louis, MO, USA) for 72 h, after which the transduction efficiency was analyzed by qRT-PCR for the target gene. 2.15 Statistical analysis Data are presented as mean ± SEM. Statistical analysis was performed using ...
1.2.2 香鱼MIP-2基因序列分析 利用巨噬细胞转录组测序获得香鱼MIP-2基因序列,采用PCR方法从香鱼巨噬细胞cDNA中扩增后测序验证,经分析所获得的序列与转录组测序结果一致( 11 )。信号肽序列预测采用SignalP 4.0在线软件(http) //.cbs.dtu.dk/services/SignalP/,。用MEGA 4.0软件( 12)进行多重序列比对和系统进化...