The tolerant group displayed relevant changes in the expression of 84 mRNA when compared to nave mice. A functional group of genes related to cell death regulation was identified. PARP-1, caspase 3, FASL and TRAIL genes were confirmed by RT-PCR to present lower expression in tolerant mice. ...
The qRT-PCR reaction was carried out using a 7900HT Fast Real-Time PCR System (Applied Biosystems). Target gene expression was normalized to Gapdh for mouse experiments and Rplp13 for human experiments and expressed as a relative value. Macrophage preparation Mouse bone marrow-derived macrophages ...
These cells were stimulated with LPS, and the expression of pro-inflammatory chemokines/cytokines that are the direct targets of KLF6 was evaluated by quantitative PCR analysis. We confirmed a reduction in BCL6 (Fig. 5G) at the protein level by Western blotting analysis. Our results indicate ...
we tried to silence APN expression by designing three short hairpin RNAs (shRNA1, shRNA2, and shRNA3). qRT-PCR and western blotting revealed a fourfold reduction in APN expression in BMDM cells treated with shRNA relative to the control group; shRNA2 was was chosen for ...
and 2 μg of purified RNA was reverse-transcribed using the GoScript Reverse Transcription System (Promega). qRT-PCR was performed with Platinum SYBR Green (Invitrogen) according to the manufacturer’s instructions on a LightCycler 480 PCR platform (Roche). The relative gene expression was determin...
The knockdown efficiency of the siRNAs in RAW264.7 macrophages or shRNAs in THP-1 macrophages was assessed by RT‒PCR and western blot analysis, respectively (Fig. 7A-B, Figure S5A-B), and the results showed that siHMGB3#1 and shHMGB3#1 had the greatest gene silencing effects. These ...
qRT-PCR confirmed reduced circATP8A1 expression, while linear ATP8A1 mRNA was not significantly altered (Fig.2A). Following the downregulation of circATP8A1, CCK-8 assay results revealed a notable decrease in the proliferative capacity (Fig.2B) and the formation of plate colonies in AGS cells...
实时荧光定量PCR结果显示,鳗利斯顿氏菌侵染香鱼后,各组织中MIP-2基因mRNA表达量均呈上调趋势。尤其以肾组织和肌肉组织中变化最显著。以上结果表明,香鱼MIP-2基因表达与鳗利斯顿氏菌的侵染密切相关,揭示了MIP-2可能在香鱼抗菌免疫反应中具有重要的作用。关键词:巨噬细胞炎性蛋白-2香鱼鳗利斯顿氏菌感染基因表达Molecular...
5d). Gene induction was analyzed by real-time quantitative PCR. Correlated with upregulated USP17 expression (Fig. 5e), stemness-associated and inflammation-associated gene expressions were upregulated when cancer cells interacted with macrophages (Fig. 5e, f), suggesting a role of USP17 in the...
Genes of interest were evaluated using the TaqMan Gene expression assay (ThermoFisher) and the TaqMan RNA-to-Ct 1-Step Kit (ThermoFisher) according to the manufacturer's instructions. Relative quantification was calculated with the standard ΔΔCt method; amplification signals from target gene ...