Modulation of M1/M2 Cytokines and Inflammatory Enzymes by Persicaria Species Leaf Extracts in Lipopolysaccharide-stimulated RAW 264.7 Cell Macrophagesdoi:10.22034/iji.2024.102221.2780Isa, Adamu ImamIranian Journal of Immunology
M1 macrophages exhibit a pro-inflammatory phenotype and produce cytokines such as tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and IL-6, critical for host defense against pathogens. 2. Alternatively activated macrophages (M2): These macrophages are induced by interleukin-4...
在病毒感染急性期,巨噬细胞向M1方向极化,M1型巨噬细胞可促进炎症反应,辅助机体清除病原体,但其过度活化可引起细胞因子风暴,加重组织的免疫病理损伤,且M1 型巨噬细胞极化比例与病情严重程度呈正相关; 随着病毒感染相关疾病的进展,巨噬细胞向M2方向极化,M2型巨噬细胞可通过分泌多种抑炎因子发挥免疫调控作用,参与组织...
Similar to the M1 story, M2 is to macrophages as Th2 is to lymphocytes; Th2 cells can produce high levels of IL-4, IL-13 and sometimes IL-10. While all three cytokines can induce M2 activation in macrophages, there are distinct differences in the resultant phenotypes, so we will concern...
【Abstract】 Objective To induce RAW264.7 cell line to M1/M2 phenotypes and identif i cate the cytokines expressed on the surface. Methods Plating the cells in 12 wells plate as 10 5 / ml 24h before intervention and use IFN-γ+LPS to induce M1 phenotype,IL-4 to induce M2 phenotype, ...
to control macrophage cell shape directly, we demonstrate here that elongation itself, without exogenous cytokines, leads to the expression of M2 phenotype markers and reduces the secretion of inflammatory cytokines. Moreover, elongation enhances the effects of M2-inducing cytokinesIL-4andIL-13and prot...
Fig. 1. Melanoma exosomes stimulate production of mixed M1 and M2 cytokines by macrophages in cell culture. (a) Induction of the M1 cytokine TNF-α in control, LPS treated, exosome treated and LPS+ exosome treated RAW 264.7 Mφs. (b) Induction of the M1 cytokine IL-1β in control, LP...
Secretome of M1 and M2 macrophages diminishes ROS synthesis by neutrophils. Neutrophils were incubated for 1 h with supernatant collected from monocytes differentiated into M1, M2a or M2c macrophages. Collected supernatants contained cytokines that were used for the polarization of macrophages, i.e. 50...
Secretome of M1 and M2 macrophages diminishes ROS synthesis by neutrophils. Neutrophils were incubated for 1 h with supernatant collected from monocytes differentiated into M1, M2a or M2c macrophages. Collected supernatants contained cytokines that were used for the polarization of macrophages, i.e. 50...
Macrophages are essential regulators of inflammation which can be classified into two major vectors of polarisation: classically activated macrophages (M1) and alternatively activated macrophages (M2). Both types of macrophages play a role in diabetes, where M1 and M2-produced cytokines can have ...