@惠生活流式m1m2圈门 惠生活 您提到的“流式m1m2圈门”似乎是一个涉及流式细胞术(Flow Cytometry)的专业术语。在流式细胞术中,M1和M2通常指的是在数据分析过程中设定的门(Gates),用于区分不同细胞亚群。圈门(Gating)是一种技术,用于在复杂的多参数数据中识别和分离特定的细胞群体。 具体解释如下: 流式细胞...
[1]巨噬细胞活化的 M1 和 M2 模式:到了需重新评估的时候了:https://www.flowcyto.cn/bbs/thread-9544-1-1.html [2]Guidelines for the use of flow cytometry and cell sorting in immunological studies:https://pubmed.ncb...
在第一版的《Guidelines for the use of flow cytometry and cell sorting in immunological studies [2]》中,曾为大家总结了人和小鼠巨噬细胞的表型特征,大家可以据此根据自己的研究需求选择: 但大家可能还是有点迷糊,不知道实际应该如何应用,没事,以人巨噬细胞为例,可参考《Regulation of Human Macrophage M1–M...
在此,将小鼠和人单核、巨噬细胞M1、M2、Kupffer细胞、DC表型汇总表分享如下,大家可根据需要,选择鉴别能力强的指标: 表格来源:Cossarizza A, Chang HD, Radbruch A, et al. Guidelines for the use of flow cytometry and cell sorting in ...
was detected by flow cytometry. Human THP-1 cells were induced by phorbol ester (PMA) to differentiate into macrophages-like cells, which were divided into M0 group, M1 group, M2 group, and M0 ESAT6 group. After 24 hours of stimulation, the mR...
正负造血调控因子的改变和巨噬细胞M1/M2亚群失衡可能是造成AA微环境损伤、最终引起AA发病的重要机制。 关键词: 贫血,再生障碍性;模型,动物;巨噬细胞;炎症介导素类 ABSTRACT ObjectiveTo investigate the role of macrophages (Mø) in the pathogenesis of modified immune-mediated aplastic anemia (AA) mice model...
目的探讨CD137-CD137L信号(简称CD137信号)是否通过调控巨噬细胞M1/M2极性转变促进血管新生。 方法将3%巯基乙酸盐肉汤诱导的小鼠腹腔原代巨噬细胞分3组,即对照组、CD137信号激动组和CD137信号抑制组,通过检测M1和M2型巨噬细胞的相关特异性标志物观察巨噬细胞表型的变化,采用流式细胞术(FCM)检测巨噬细胞表面CD137、...
结论激活PPAR-γ受体可以促进M1型THP-1巨噬细胞向M2型转化,降低炎症因子分泌以及巨噬细胞的脂质沉积。 关键词: 过氧化物酶体增殖物激活受体-γ;巨噬细胞极化;脂质代谢 ABSTRACT ObjectiveTo investigate the effect of peroxisome proliferator-activated receptor-γ(PPAR-γ) on the polarization and lipid metabolism...
Inhibiting CISD1 expression promotes macrophage polarization towards the M1 phenotype and inhibits M2 phenotype polarization. A Screening of si-CISD1 sequences, followed by Western Blot to detect CISD1 protein expression levels. B Detection of M1/M2 polarization using flow cytometry after si-CISD1 tr...
The release of neutrophil extracellular traps (NETs) can be either beneficial or detrimental for the host, thus it is necessary to maintain a balance between formation and clearance of NETs. Multiple physiological factors eliciting NET release have been