If the lysis efficiency for the specific cell line is not optimal, we suggest increasing the time of incubation and the amount of M-PER reagent used. It can also help to shake the samples more vigorously during the incubation.Find additional tips, troubleshooting help, and resources within our...
FM2 cells were transiently transfected with a reporter construct containing the luciferase gene. The transfected cells were lysed with either M-PER Reagent or Brand P lysis buffer and subjected to luciferase assay. For β-galactosidase and CAT assays, MDA-MB-231 cells were contransfected with repor...
We also offer the Pierce IP Lysis buffer (Cat. Nos. 87787 and 87788) as well as M-PER (Cat. Nos. 78501, 78503, and 78505). Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center. Please see the various lysis buffers we offer (...
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lysis buffer (25 mM HEPES, 500 mM NaCl; 2 mM phenylmethylsulphonyl fluoride (PMSF), 10 mg/ml lysozyme, pH 7.4) in a volume equivalent to the initial culture volume. Lysis of bacteria was facilitated by sonication on ice at 200 Hz for 20 s for 10 cycles. Then, 100 µL of the ...
Plates were washed 5 times with RPMI-1640 media before the addition of 200 μl of 1:5 dilution of lysis buffer in RPMI-1640 media (p24 kit, ABL Inc.). Samples were stored at −80 °C until assayed for p24 by ELISA according to the manufacturer's instructions. The binding of the ...
In complement-dependent cytotoxicity (CDC), antibody binding to the target cell induces cell lysis that is triggered by the binding of C1q subunits to the Fc antibody region. For in vitro ADCC evaluations, CEM-NKR-CCR5 CD4 T cells infected with one of four HIV-1 viral strains were cultured...
Thermo Scientific M-PER哺乳动物蛋白质抽提试剂用于从培养的哺乳动物细胞中高效抽提总可溶性蛋白。M-PER哺乳动物蛋白抽提试剂的特点:• 作用温和— 温和的除垢剂裂解,产生的抽提物直接与考马斯(Bradford)和BCA蛋白定量分析试剂或SDS-PAGE兼容• 兼容性强— 抽提的可溶性蛋白处于非变性状态,可直接用于免疫沉淀反应和...
Penetration of sperm through the thick outer layer of the ovum is achieved through chemical lysis of the ovum coat and/or the mechanical motion of sperm (Bedford (1998), Biol Reprod 59, 1275-87). Following infiltration of the ovum ZP coat, the sperm membrane fuses with that of ovum. ...